公开(公告)号：US20070009893A1

公开(公告)日：2007-01-11

申请号：US10568101

申请日：2004-09-08

申请人： Toshihiro Mori ,  Yoshihiko Maniko ,  Rie Hando ,  Yumiko Takeshita ,  Hiroko Inomata

发明人： Toshihiro Mori ,  Yoshihiko Maniko ,  Rie Hando ,  Yumiko Takeshita ,  Hiroko Inomata

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1006 ,  C12N15/1017

摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.

摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。