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    Method for labeled amidation of biological molecule and method for analyzing biological molecule
    2.
    发明申请
    Method for labeled amidation of biological molecule and method for analyzing biological molecule 审中-公开
    生物分子的标记酰胺化方法和生物分子分析方法

    公开(公告)号：US20050089951A1

    公开(公告)日：2005-04-28

    申请号：US10969900

    申请日：2004-10-22

    申请人： Koichi Tanaka ,  Sadanori Sekiya ,  Yoshinao Wada

    发明人： Koichi Tanaka ,  Sadanori Sekiya ,  Yoshinao Wada

    IPC分类号： A61K51/00 ,  A61K51/08 ,  C07K1/13 ,  C07K14/805 ,  C12P21/06 ,  G01N33/60

    CPC分类号： C07K14/805 ,  A61K51/0491 ,  C07K1/13 ,  G01N33/60 ,  G01N2458/15

    摘要： The present invention provides a method for improving the sensitivity of mass spectrometry for biological molecules. The present invention also provides a method for a rapid and simple analysis of biological samples by making use of the method for improving the sensitivity of mass spectrometry. A method for labeled amidation, comprising the step of reacting an amidating reagent labeled with 15N, an isotope of nitrogen, with the carboxyl group of a biological molecule. A method for analyzing a biological molecule, comprising the steps of performing amidation to a carboxyl group of a biological molecule to obtain an amidated form of the biological molecule, and subsequently subjecting the amidated form of the biological molecule to mass spectrometry. A labeled amidated form of a biological molecule wherein a carboxyl group of the biological molecule is amidated as 15N-labeled amide group.

    摘要翻译： 本发明提供了提高生物分子质谱的灵敏度的方法。 本发明还提供了通过利用提高质谱灵敏度的方法快速简单地分析生物样品的方法。 一种用于标记酰胺化的方法，包括使标示有氮的同位素氮的酰胺化试剂与生物分子的羧基反应的步骤。 一种用于分析生物分子的方法，包括以下步骤：对生物分子的羧基进行酰胺化以获得酰胺化形式的生物分子，随后使酰胺化形式的生物分子进行质谱分析。 生物分子的标记酰胺化形式，其中生物分子的羧基被酰胺化为“N”标记的酰胺基。

    Sample target used in mass spectrometry, method for producing the same, and mass spectrometer using the sample target
    3.
    发明申请
    Sample target used in mass spectrometry, method for producing the same, and mass spectrometer using the sample target 失效
    用于质谱的样品靶，其制备方法和使用样品靶的质谱仪

    公开(公告)号：US20090095897A1

    公开(公告)日：2009-04-16

    申请号：US11988166

    申请日：2006-04-28

    申请人： Shoji Okuno ,  Yoshinao Wada ,  Takashi Yanagishita ,  Hideki Masuda

    发明人： Shoji Okuno ,  Yoshinao Wada ,  Takashi Yanagishita ,  Hideki Masuda

    IPC分类号： B01D59/44 ,  H01J49/00

    CPC分类号： H01J49/0418

    摘要： In one embodiment of the present invention, a sample target that allows ionization of a substance whose molecular weight is so high as to exceed 10000 in mass spectrometry that ionizes a sample without using matrix, a method for producing the same and a mass spectrometer using the sample target. The sample target includes a sample support surface including a large number of fine pores on its face receiving irradiated laser light. Each of the fine pores has a diameter of 30 nm or more and 5 μm or less. The number indicative of pore depth/(pore cycle−pore diameter) of each of the fine pores is 2 or more and 50 or less. The face of the sample support surface is coated with metal or semiconductor.

    摘要翻译： 在本发明的一个实施方式中，能够使分子量高达10000以上的物质离子化的样品靶，该质谱在不使用基质的情况下离子化样品，其制造方法和质谱仪 样本目标 样品靶包括在其表面上接收照射的激光的大量细孔的样品支撑表面。 每个细孔的直径为30nm以上且5μm以下。 每个细孔的孔深度（孔隙孔径）的数量为2以上且50以下。 样品支撑表面的表面涂有金属或半导体。

    Sample target used in mass spectrometry, method for producing the same, and mass spectrometer using the sample target
    4.
    发明授权
    Sample target used in mass spectrometry, method for producing the same, and mass spectrometer using the sample target 失效
    用于质谱的样品靶，其制备方法和使用样品靶的质谱仪

    公开(公告)号：US08237114B2

    公开(公告)日：2012-08-07

    申请号：US11988166

    申请日：2006-04-28

    申请人： Shoji Okuno ,  Yoshinao Wada ,  Takashi Yanagishita ,  Hideki Masuda

    发明人： Shoji Okuno ,  Yoshinao Wada ,  Takashi Yanagishita ,  Hideki Masuda

    IPC分类号： H01J49/26

    CPC分类号： H01J49/0418

    摘要： In one embodiment of the present invention, a sample target that allows ionization of a substance whose molecular weight is so high as to exceed 10000 in mass spectrometry that ionizes a sample without using matrix, a method for producing the same and a mass spectrometer using the sample target. The sample target includes a sample support surface including a large number of fine pores on its face receiving irradiated laser light. Each of the fine pores has a diameter of 30 nm or more and 5 μm or less. The number indicative of pore depth/(pore cycle−pore diameter) of each of the fine pores is 2 or more and 50 or less. The face of the sample support surface is coated with metal or semiconductor.

    摘要翻译： 在本发明的一个实施方式中，能够使分子量高达10000以上的物质离子化的样品靶，该质谱在不使用基质的情况下离子化样品，其制造方法和质谱仪 样本目标 样品靶包括在其表面上接收照射的激光的大量细孔的样品支撑表面。 每个细孔的直径为30nm以上且5μm以下。 每个细孔的孔深度（孔隙孔径）的数量为2以上且50以下。 样品支撑表面的表面涂有金属或半导体。