公开(公告)号：US08039223B2

公开(公告)日：2011-10-18

申请号：US12468440

申请日：2009-05-19

申请人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

发明人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

IPC分类号： G01N33/53

CPC分类号： G01N33/74 ,  G01N2800/042 ,  G01N2800/32

摘要： Kits and methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.

摘要翻译： 用于选择性测定生物样品中目标脂联素多聚体的试剂盒和方法。 这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。 一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其它脂联素多聚体区分开。

公开(公告)号：US20080044926A1

公开(公告)日：2008-02-21

申请号：US11793980

申请日：2005-12-22

申请人： Yuki Honjo ,  Yuka Akimoto ,  Kazuo Kotani ,  Hirokazu Yago ,  Masanao Matsuo ,  Osamu Miyazaki ,  Kazunori Saito

发明人： Yuki Honjo ,  Yuka Akimoto ,  Kazuo Kotani ,  Hirokazu Yago ,  Masanao Matsuo ,  Osamu Miyazaki ,  Kazunori Saito

IPC分类号： G01N33/566

CPC分类号： G01N33/54333 ,  G01N33/54313 ,  G01N33/57434

摘要： It is intended to provide a reagent and an assay method whereby an antigen can be conveniently and accurately assayed without resorting to any special antibody. Using two or more carriers having different particle sizes, a carrier having a smaller particle size is sensitized with at least one monoclonal antibody, which is selected from among three monoclonal antibodies being reactive with both of a free antigen and a complex antigen and having different recognition sites from each other, while a carrier having a larger particle size is sensitized with the remainder monoclonal antibodies, thereby providing an assay reagent and an assay method whereby the reactivities of a free antigen and a complex antigen can be controlled.

摘要翻译： 旨在提供一种试剂和测定方法，其中可以方便和准确地测定抗原而不诉诸任何特殊的抗体。 使用两种或更多种具有不同粒度的载体，具有较小粒度的载体用至少一种单克隆抗体敏化，所述单克隆抗体选自与游离抗原和复合抗原两者具有不同识别性的三种单克隆抗体 而具有较大粒径的载体则与剩余的单克隆抗体敏感，从而提供测定试剂和测定方法，由此可以控制游离抗原和复合抗原的反应性。

公开(公告)号：US07781171B2

公开(公告)日：2010-08-24

申请号：US10575711

申请日：2004-10-15

申请人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi

发明人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi

IPC分类号： G01N33/53

CPC分类号： G01N33/74 ,  G01N2333/72 ,  Y10T436/25

摘要： The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers.The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.

摘要翻译： 本发明提供一种方法，用于方便，快速和准确地测量存在于受各种脂联素多聚体污染的生物样品中的脂连蛋白的总量的样品。 用于免疫测定样品中存在的脂联素总量的样品的测定方法包括使含有脂联素的样品与还原剂，其酸或其盐，表面活性剂和蛋白酶中的至少一种反应。

公开(公告)号：US07608405B2

公开(公告)日：2009-10-27

申请号：US10575931

申请日：2004-10-15

申请人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

发明人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

IPC分类号： G01N33/53 ,  G01N33/543 ,  A61K39/395

CPC分类号： G01N33/74 ,  G01N2800/042 ,  G01N2800/32

摘要： Methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying of a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.

摘要翻译： 用于选择性测定生物样品中目标脂联素多聚体的方法。 这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。 一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其他脂联素多聚体区分开。

公开(公告)号：US20070042424A1

公开(公告)日：2007-02-22

申请号：US10575931

申请日：2004-10-15

申请人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

发明人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

IPC分类号： G01N33/53 ,  C12Q1/37

CPC分类号： G01N33/74 ,  G01N2800/042 ,  G01N2800/32

摘要： We have discovered a “human adiponectin ELISA kit”, which can specifically measure adiponectin in human blood serum (or blood plasma) or in extracted fluid from lipocytes or culture supernatant fluid. After fractioning human serum by use of gel filtration column, each fraction was measured by the present kit, and as a result, adiponectin immunity activity was detected as a plurality of peaks at above 670 kD of molecular weight. From the results, it is presumed that adiponectin is present in the blood as a polymer or forms a complex with polymer proteins.

摘要翻译： 我们已经发现了一种“人类脂联素ELISA试剂盒”，可以特异性地测量人血清（或血浆）中的脂联素或来自脂肪细胞或培养上清液的提取液。 通过使用凝胶过滤柱对人血清进行分馏后，通过本试剂盒测定各级分，结果，在670kD以上的分子量下检测出多个峰的脂联素免疫活性。 从结果可以推测，脂联素作为聚合物存在于血液中，或与聚合物蛋白形成复合物。

公开(公告)号：US20070065891A1

公开(公告)日：2007-03-22

申请号：US10575711

申请日：2004-10-15

申请人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi

发明人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi

IPC分类号： G01N33/569 ,  G01N33/554

CPC分类号： G01N33/74 ,  G01N2333/72 ,  Y10T436/25

摘要： The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers. The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.

摘要翻译： 本发明提供一种方法，用于方便，快速和准确地测量存在于受各种脂联素多聚体污染的生物样品中的脂连蛋白的总量的样品。 用于免疫测定样品中存在的脂联素总量的样品的测定方法包括使含有脂联素的样品与还原剂，其酸或其盐，表面活性剂和蛋白酶中的至少一种反应。

公开(公告)号：US20090291461A1

公开(公告)日：2009-11-26

申请号：US12468440

申请日：2009-05-19

申请人： Hiroyuki EBINUMA ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

发明人： Hiroyuki EBINUMA ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

IPC分类号： G01N33/53

CPC分类号： G01N33/74 ,  G01N2800/042 ,  G01N2800/32

摘要： Kits and methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.

摘要翻译： 用于选择性测定生物样品中目标脂联素多聚体的试剂盒和方法。 这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。 一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其它脂联素多聚体区分开。