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    Method of selectively assaying adiponectin multimers
    1.
    发明授权
    Method of selectively assaying adiponectin multimers 有权
    选择性测定脂连蛋白多聚体的方法

    公开(公告)号：US08039223B2

    公开(公告)日：2011-10-18

    申请号：US12468440

    申请日：2009-05-19

    申请人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

    发明人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

    IPC分类号： G01N33/53

    CPC分类号： G01N33/74 ,  G01N2800/042 ,  G01N2800/32

    摘要： Kits and methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.

    摘要翻译： 用于选择性测定生物样品中目标脂联素多聚体的试剂盒和方法。 这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。 一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其它脂联素多聚体区分开。

    Method of selectively assaying adiponectin multimers
    3.
    发明申请
    Method of selectively assaying adiponectin multimers 有权
    选择性测定脂连蛋白多聚体的方法

    公开(公告)号：US20070042424A1

    公开(公告)日：2007-02-22

    申请号：US10575931

    申请日：2004-10-15

    申请人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

    发明人： Hiroyuki Ebinuma ,  Hirokazu Yago ,  Yuka Akimoto ,  Osamu Miyazaki ,  Takashi Kadowaki ,  Toshimasa Yamauchi ,  Kazuo Hara

    IPC分类号： G01N33/53 ,  C12Q1/37

    CPC分类号： G01N33/74 ,  G01N2800/042 ,  G01N2800/32

    摘要： We have discovered a “human adiponectin ELISA kit”, which can specifically measure adiponectin in human blood serum (or blood plasma) or in extracted fluid from lipocytes or culture supernatant fluid. After fractioning human serum by use of gel filtration column, each fraction was measured by the present kit, and as a result, adiponectin immunity activity was detected as a plurality of peaks at above 670 kD of molecular weight. From the results, it is presumed that adiponectin is present in the blood as a polymer or forms a complex with polymer proteins.

    摘要翻译： 我们已经发现了一种“人类脂联素ELISA试剂盒”，可以特异性地测量人血清（或血浆）中的脂联素或来自脂肪细胞或培养上清液的提取液。 通过使用凝胶过滤柱对人血清进行分馏后，通过本试剂盒测定各级分，结果，在670kD以上的分子量下检测出多个峰的脂联素免疫活性。 从结果可以推测，脂联素作为聚合物存在于血液中，或与聚合物蛋白形成复合物。

    Anti-human soluble fibrin monoclonal antibody and immunological assay method using the antibody
    6.
    发明授权
    Anti-human soluble fibrin monoclonal antibody and immunological assay method using the antibody 有权
    抗人溶性纤维蛋白单克隆抗体和免疫测定法使用抗体

    公开(公告)号：US07829294B2

    公开(公告)日：2010-11-09

    申请号：US11722998

    申请日：2005-12-27

    申请人： Masanao Matsuo ,  Hiroyuki Ebinuma ,  Osamu Miyazaki ,  Kyoko Tanaka ,  Akiko Suzuki

    发明人： Masanao Matsuo ,  Hiroyuki Ebinuma ,  Osamu Miyazaki ,  Kyoko Tanaka ,  Akiko Suzuki

    IPC分类号： G01N33/543 ,  G01N33/573 ,  G01N33/577 ,  C07K16/36 ,  C12N5/20 ,  C12P21/08

    CPC分类号： C07K16/18 ,  C07K14/745 ,  C07K14/75 ,  G01N33/86 ,  G01N2333/75 ,  G01N2800/224

    摘要： The present invention is directed to a monoclonal antibody against a soluble fibrin, which specifically recognizes a conformation-changed site newly occurred in a C-terminal region of an Aα-chain of the soluble fibrin formed through thrombin digestion of fibrinogen. The present invention is also directed to a hybridoma which produces the antibody, an immunological assay method employing the antibody, and a method for evaluating hypercoagulability in a test sample by measuring the soluble fibrin level in the sample with the assay method. Through employment of the monoclonal antibody of the present invention, soluble fibrin on which plasmin has not acted, which reflects exclusively initial hypercoagulability, can be specifically detected.

    摘要翻译： 本发明涉及针对可溶性纤维蛋白的单克隆抗体，其特异性识别通过纤维蛋白原凝血酶消化形成的可溶性纤维蛋白的Aα链的C末端区域中新发生的构象改变部位。 本发明还涉及产生抗体的杂交瘤，使用抗体的免疫测定方法，以及通过使用测定方法测量样品中的可溶性纤维蛋白水平来评估测试样品中的高凝状态的方法。 通过使用本发明的单克隆抗体，可以特异性检测仅反映初始高凝状态的纤维蛋白溶酶未起作用的可溶性纤维蛋白。

    Anti-Human Soluble Fibrin Monoclonal Antibody and Immunilogical Assay Method Using the Antibody
    7.
    发明申请
    Anti-Human Soluble Fibrin Monoclonal Antibody and Immunilogical Assay Method Using the Antibody 有权
    抗人溶纤维蛋白单克隆抗体和使用抗体的免疫测定方法

    公开(公告)号：US20080009077A1

    公开(公告)日：2008-01-10

    申请号：US11722998

    申请日：2005-12-27

    申请人： Masanao Matsuo ,  Hiroyuki Ebinuma ,  Osamu Miyazaki ,  Kyoko Tanaka ,  Akiko Suzuki

    发明人： Masanao Matsuo ,  Hiroyuki Ebinuma ,  Osamu Miyazaki ,  Kyoko Tanaka ,  Akiko Suzuki

    IPC分类号： G01N33/566 ,  C07K16/36 ,  C12N5/12

    CPC分类号： C07K16/18 ,  C07K14/745 ,  C07K14/75 ,  G01N33/86 ,  G01N2333/75 ,  G01N2800/224

    摘要： The present invention is directed to a monoclonal antibody against a soluble fibrin, which specifically recognizes a conformation-changed site newly occurred in a C-terminal region of an Aα-chain of the soluble fibrin formed through thrombin digestion of fibrinogen. The present invention is also directed to a hybridoma which produces the antibody, an immunological assay method employing the antibody, and a method for evaluating hypercoagulability in a test sample by measuring the soluble fibrin level in the sample with the assay method. Through employment of the monoclonal antibody of the present invention, soluble fibrin on which plasmin has not acted, which reflects exclusively initial hypercoagulability, can be specifically detected.

    摘要翻译： 本发明涉及针对可溶性纤维蛋白的单克隆抗体，其特异性识别通过纤维蛋白原的凝血酶消化形成的可溶性纤维蛋白的Aalpha链的C末端区域中新发生的构象改变部位。 本发明还涉及产生抗体的杂交瘤，使用抗体的免疫测定方法，以及通过使用测定方法测量样品中的可溶性纤维蛋白水平来评估测试样品中的高凝状态的方法。 通过使用本发明的单克隆抗体，可以特异性检测仅反映初始高凝状态的纤维蛋白溶酶未起作用的可溶性纤维蛋白。

    METHOD FOR IMMUNOLOGICALLY MEASURING SOLUBLE LR11
    8.
    发明申请
    METHOD FOR IMMUNOLOGICALLY MEASURING SOLUBLE LR11 有权
    免疫测定方法LR11

    公开(公告)号：US20140080158A1

    公开(公告)日：2014-03-20

    申请号：US14116683

    申请日：2012-05-09

    申请人： Kohei Takubo ,  Hiroyuki Ebinuma ,  Isamu Fukamachi ,  Hideaki Bujo ,  Yasushi Saito

    发明人： Kohei Takubo ,  Hiroyuki Ebinuma ,  Isamu Fukamachi ,  Hideaki Bujo ,  Yasushi Saito

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54393 ,  G01N33/92

    摘要： To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample).The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.

    摘要翻译： 为了提供用于测定生物样品中可溶性LR11的方法，该方法通过免疫学手段实现了样品中存在的可溶性LR11的简单且准确的测定，而不需要从生物样品（例如血清样品）分离可溶性LR11。 用于免疫测定存在于生物样品中的可溶性LR11的本发明的方法，其特征在于所述方法包括用选自一种或多种磺基甜菜碱两性表面活性剂和一种或多种氨基磺基甜菜碱两性表面活性剂中的至少一种表面活性剂处理样品。

    Method for quantitatively determining a reducing substance and a reagent for quantitative determination
    9.
    发明授权
    Method for quantitatively determining a reducing substance and a reagent for quantitative determination 有权
    用于定量测定还原物质和定量测定试剂的方法

    公开(公告)号：US07244620B2

    公开(公告)日：2007-07-17

    申请号：US10743741

    申请日：2003-12-24

    申请人： Masahiro Sekiguchi ,  Takuji Matsumoto ,  Hiroyuki Ebinuma

    发明人： Masahiro Sekiguchi ,  Takuji Matsumoto ,  Hiroyuki Ebinuma

    IPC分类号： G01N33/00 ,  G01N21/00 ,  G01N21/75 ,  C12Q1/00 ,  C12P11/00 ,  C12N9/88

    CPC分类号： G01N31/22 ,  Y10T436/18 ,  Y10T436/182

    摘要： The present invention provides a method for quantitatively determining a reducing substance, which comprises reacting a reducing substance in a test specimen with iron (III) ions, reacting iron (II) ions formed by reduction of the iron (III) ions or residual iron (III) ions with a metal indicator which is capable of reacting specifically with the iron (II) ions or the residual iron (III) ions to undergo color development, and carrying out quantitative determination by measuring the degree of color development, wherein a chelating agent which is specific to copper ions is added to the test specimen before the reaction of the reducing substance with the iron (III) ions; and a reagent used for it.

    摘要翻译： 本发明提供了一种定量测定还原物质的方法，该方法包括使试样中的还原物质与铁（III）离子反应，通过还原铁（III）离子或残余铁形成的铁（II） III）离子与能够与铁（II）离子或残留铁（III）离子特异性反应的金属指示剂进行显色，并通过测量显色程度进行定量测定，其中螯合剂 在还原物质与铁（III）离子反应之前，将铜离子特异性添加到试样中; 和用于它的试剂。

    Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine
    10.
    发明授权
    Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine 有权
    用于定量测定同型半胱氨酸的方法和用于定量测定同型半胱氨酸的试剂

    公开(公告)号：US07198890B2

    公开(公告)日：2007-04-03

    申请号：US10755236

    申请日：2004-01-13

    申请人： Hiroyuki Ebinuma ,  Takami Sarashina ,  Takuji Matsumoto ,  Masahiro Sekiguchi ,  Koji Ushizawa

    发明人： Hiroyuki Ebinuma ,  Takami Sarashina ,  Takuji Matsumoto ,  Masahiro Sekiguchi ,  Koji Ushizawa

    IPC分类号： C12Q1/00 ,  C12N9/00 ,  C07K1/00

    CPC分类号： G01N33/6812 ,  G01N2333/90241

    摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.

    摘要翻译： 本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在 不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度 硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。