公开(公告)号：US5606116A

公开(公告)日：1997-02-25

申请号：US550467

申请日：1995-10-30

申请人： Yuko Yoneda ,  Toshiyuki Itooka

发明人： Yuko Yoneda ,  Toshiyuki Itooka

IPC分类号： G01N33/52 ,  A01N1/02 ,  C12Q1/68

CPC分类号： G01N33/521 ,  Y10T436/171538

摘要： A testing system for urinalysis is provided comprising a support member which carries a developing phase, a reagent carrying means, a urine applying section, and a urine-reagent developing area. In order to prevent inaccurate test results which may result from splashing the sample onto the device, a water-repellent agent is coated onto both sides of the reagent phase and the urine-reagent developing phase, as well as onto both sides of the support member where the reagent phase and the urine-reagent developing phase are provided. The water repellent agent is solidified, and a transparent resin film is attached to the upper surfaces of the reagent phase and the urine-reagent developing phase. In another embodiment, instead of the combination of a water repellent agent and a transparent resin film attached to the upper surfaces of the reagent phase and the urine-reagent developing phase, the device is enclosed in an envelope-like or cylindrical transparent resin film or container.

摘要翻译： 提供了一种用于尿分析的测试系统，其包括携带显影期的支撑构件，试剂携带装置，尿液施加部分和尿液显影区域。 为了防止将样品溅到装置上可能导致的不精确的测试结果，将防水剂涂覆在试剂相和尿液显影阶段的两侧以及涂覆在支撑构件的两侧上 其中提供了试剂相和尿液显影阶段。 防水剂固化，透明树脂膜附着在试剂相和尿液显影相的上表面。 在另一个实施方案中，代替安装在试剂相和尿液显影相的上表面上的防水剂和透明树脂膜的组合，该装置被封装在包封状或圆柱形的透明树脂膜中，或 容器。

公开(公告)号：US4801541A

公开(公告)日：1989-01-31

申请号：US58688

申请日：1987-06-03

申请人： Yuko Yoneda ,  Frank E. Young

发明人： Yuko Yoneda ,  Frank E. Young

IPC分类号： C12N9/28 ,  C12N15/00 ,  C12N1/20 ,  C12R1/125

CPC分类号： C12N9/2417 ,  C12N15/00 ,  Y10S435/839

摘要： A method is described for increasing the yield of a product from a microorganism containing a regulatory gene, by altering the microorganism. The method involves introducing into the microorganism at least one structural gene for the product by lysogenizing the microorganism with a recombinant bacteriophage containing the structural gene.

摘要翻译： 描述了通过改变微生物从含有调节基因的微生物中提高产物产量的方法。 该方法包括通过用含有结构基因的重组噬菌体溶解微生物来向微生物引入至少一种产物结构基因。

公开(公告)号：US4886754A

公开(公告)日：1989-12-12

申请号：US740312

申请日：1985-06-03

申请人： Richard S. Graham ,  Yuko Yoneda ,  Frank E. Young

发明人： Richard S. Graham ,  Yuko Yoneda ,  Frank E. Young

IPC分类号： C12N7/01 ,  C12N9/28 ,  C12N15/75

CPC分类号： C12N9/2417 ,  C12N15/75 ,  Y10S435/832 ,  Y10S435/839

摘要： A recombinant bacteriophage, a method for producing and selecting the recombinant bacteriophage and a method for heterologous cloning of DNA are disclosed. The recombinant bacteriophage is produced by ligating genetic fragments encoding a desired genetic trait with DNA from a bacteriophage, incubating with DNA from a second Bacillus microorganism prototrophic for a growth requirement, incubating with a host Bacillus auxotrophic for the growth requirement. Transformed host Bacillus are selected by growing the mixture on a growth medium which does not contain the growth requirement and determining the presence of the genetic trait. The recombinant bacteriophage containing the desired genetic trait is recovered from the host Bacillus by induction. Heterologous cloning can be accomplished by incubating a host Bacillus with the recombinant bacteriophage.

摘要翻译： 公开了重组噬菌体，重组噬菌体的制备和选择方法以及异源克隆DNA的方法。 通过将编码期望的遗传性状的遗传片段与来自噬菌体的DNA连接起来，与来自第二种芽孢杆菌微生物原生质体的DNA一起孵育以用于生长需求，通过与宿主芽孢杆菌营养缺陷型孵育用于生长需要来产生重组噬菌体。 通过在不含生长需要并确定遗传性状的存在的生长培养基上生长混合物来选择转化的宿主芽孢杆菌。 通过诱导从宿主芽孢杆菌中回收含有所需遗传特征的重组噬菌体。 可以通过将宿主芽孢杆菌与重组噬菌体一起孵育来实现异源克隆。