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    DNA helicase
    1.
    发明授权
    DNA helicase 失效
    DNA解旋酶

    公开(公告)号：US06939699B2

    公开(公告)日：2005-09-06

    申请号：US10373090

    申请日：2003-02-26

    申请人： Ikuo Matsui ,  Hiroki Higashibata ,  Yutaka Kawarabayashi

    发明人： Ikuo Matsui ,  Hiroki Higashibata ,  Yutaka Kawarabayashi

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/90 ,  C12N15/61 ,  C12N9/10 ,  C12N9/14

    CPC分类号： C12N9/90

    摘要： The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such as Escherichia coli in the usual manner to create a transformant, and culturing the transformant.

    摘要翻译： 本发明提供具有DNA解旋酶活性的热稳定酶，优选结构特异性内切核酸酶活性。 该酶可以通过将编码该酶的基因从超嗜热菌，优选硫代谢嗜热古细菌的染色体DNA克隆到表达载体中，将该基因导入表达载体中，将载体以常规方式引入宿主如大肠杆菌 创造转化体，培养转化体。

    Method for modifying a property of a protein
    2.
    发明申请
    Method for modifying a property of a protein 有权
    改变蛋白质性质的方法

    公开(公告)号：US20050233308A1

    公开(公告)日：2005-10-20

    申请号：US10933280

    申请日：2004-09-03

    申请人： Yosuke Nishio ,  Eiichiro Kimura ,  Yoshihiro Usuda ,  Kazuho Ikeo ,  Yoji Nakamura ,  Takashi Gojobori ,  Yutaka Kawarabayashi ,  Yumi Hino ,  Eiichi Hori ,  Jun Yamazaki

    发明人： Yosuke Nishio ,  Eiichiro Kimura ,  Yoshihiro Usuda ,  Kazuho Ikeo ,  Yoji Nakamura ,  Takashi Gojobori ,  Yutaka Kawarabayashi ,  Yumi Hino ,  Eiichi Hori ,  Jun Yamazaki

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/00 ,  C12N15/10 ,  C12P21/02 ,  C12Q1/00 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

    CPC分类号： C12N9/88 ,  C12N9/00 ,  C12N9/0016 ,  C12N9/1205 ,  C12N15/1034 ,  C12N15/1089

    摘要： A property of a protein is modified by the following steps: (a) selecting 1000 or more genes from the genome of a first microorganism, and selecting 1000 or more genes from the genome of a second microorganism, wherein the genes from the first microorganism are orthologs to the genes from the second microorganism, and wherein the second microorganism is closely related to the first microorganism, but grows differently under at least one optimum growth condition when compared with the first microorganism, (b) comparing an amino acid sequence encoded by a gene from the first microorganism to an amino acid sequence encoded by the orthologous gene from the second microorganism, (c) detecting substitutions between the amino acid sequence encoded by a gene from the first microorganism and the amino acid sequence encoded by a gene from the second microorganism for each pair of orthologous genes, (d) compiling the detected amino acid substitutions for each amino acid substitution type, (e) calculating the frequency of each amino acid substitution type, wherein for each detected amino acid substitution type, a correction is made by subtracting the total number of substitution types which occur from the first microorganism to the second microorganism from the total number of the same substitution type which occurs in the reverse direction, or from the second microorganism to the first microorganism, (f) identifying and labelling the amino acid substitutions which occur at a high frequency as amino acid substitutions which are involved in said optimum growth condition, and (g) introducing one or more of the amino acid substitutions identified in (f) into the gene encoding the protein to modify a property of the protein.

    摘要翻译： 通过以下步骤修饰蛋白质的性质：（a）从第一微生物的基因组中选择1000个或更多个基因，并从第二微生物的基因组中选择1000个或更多个基因，其中来自第一个微生物的基因是 与第二微生物的基因直系同源，其中第二微生物与第一微生物密切相关，但与第一微生物相比，在至少一个最佳生长条件下生长不同，（b）比较由第一微生物编码的氨基酸序列 基因从第一微生物到由第二微生物的直系同源基因编码的氨基酸序列，（c）检测由第一微生物的基因编码的氨基酸序列与由第二微生物的基因编码的氨基酸序列之间的取代 每对直系同源基因的微生物，（d）编码每个氨基酸取代类型的检测到的氨基酸取代，（e）cal 规定每个氨基酸取代型的频率，其中对于每个检测到的氨基酸取代类型，通过从相同的取代类型的总数中减去从第一微生物发生到第二微生物的替代类型的总数来进行校正 （f）鉴定和标记高频发生的氨基酸取代作为参与所述最佳生长条件的氨基酸取代，和（g） 将（f）中鉴定的一个或多个氨基酸取代引入编码蛋白质的基因中以改变蛋白质的性质。