公开(公告)号：US09807986B2

公开(公告)日：2017-11-07

申请号：US15083269

申请日：2016-03-28

Applicant: Rui Hou ,  Yuwei Wang ,  Huifang Liang ,  Zhanguo Zhang ,  Zhimin Liu ,  Binhao Zhang ,  Bixiang Zhang ,  Xiaoping Chen

Inventor： Rui Hou ,  Yuwei Wang ,  Huifang Liang ,  Zhanguo Zhang ,  Zhimin Liu ,  Binhao Zhang ,  Bixiang Zhang ,  Xiaoping Chen

IPC: A01K67/027 ,  A61K49/00 ,  G01N33/50 ,  C12N5/09 ,  C12N9/02

CPC classification number: A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  C12N5/0693 ,  C12N9/0069 ,  C12Y113/12007

Abstract: A method for establishing an animal model of hepatocellular carcinoma (HCC) bone metastasis, the method including: 1) establishing 97H and LM3 cell clones with stable expression of firefly luciferase (LUC); 2) allowing HCC cells to form bone metastasis in nude mice via intratibial injection; 3) reproducing HCC bone metastasis in nude mice via intracardiac injection of tumor cells; and 4) isolating a sub-population of tumor cells that targets metastasis to bone. The 97H and the LM3 are highly metastatic HCC cell lines transfected with luciferase gene. BALB/cA-nu mice are 4-5 weeks old and maintained in laminar flow cabinets under SPF conditions and received human care throughout an entire study. A cell number for intratibial injection is 0.5×106, and a cell number for intracardiac injection is 1×106.

公开(公告)号：USD678582S1

公开(公告)日：2013-03-19

申请号：US29413251

申请日：2012-02-13

Applicant: Jifang Du ,  Wei Wang ,  Jinhua Zhang ,  Yuwei Wang

Designer： Jifang Du ,  Wei Wang ,  Jinhua Zhang ,  Yuwei Wang

公开(公告)号：US08746157B2

公开(公告)日：2014-06-10

申请号：US13407785

申请日：2012-02-29

Applicant: Yuwei Wang ,  Zhenqiang Liu ,  Jing Zhang ,  Qian Yu

Inventor： Yuwei Wang ,  Zhenqiang Liu ,  Jing Zhang ,  Qian Yu

IPC: F23J1/00 ,  F23J1/02 ,  C10J3/34

CPC classification number: F23J1/02

Abstract: A bottom ash discharging device for a coal-fired boiler including: a clinker case, installed between a bottom ash hopper outlet of the coal-fired boiler and a bottom ash conveyer, an impact bar including a plurality of heat-resistant metal rods supporting inside the clinker case, a clinker crusher, installed above the impact bar for crushing the clinker, and a guiding device, installed between the bottom ash hopper outlet and the clinker crusher for limiting the clinker to drop into the clinker crushing area of the clinker crusher. The bottom ash discharging device ensures that the clinkers drop from the bottom ash hopper outlet to the rear area of the clinker crusher, and that the impact bar can be replaced and repaired without stopping the coal-fired boiler.

Abstract translation: 一种用于燃煤锅炉的底灰排放装置，包括：安装在燃煤锅炉的底灰料斗出口和底灰输送机之间的熟料箱，包括多个支撑在内部的耐热金属棒的冲击杆 熟料盒，熟料破碎机，安装在冲击棒上方，用于破碎熟料;以及引导装置，安装在底灰斗出料口和熟料破碎机之间，用于限制熟料落入熟料破碎机的熟料破碎区域。 底灰排放装置可确保熟料从底灰料斗出口落到熟料破碎机的后部区域，并可在不停止燃煤锅炉的情况下更换和修理冲击棒。

公开(公告)号：US20120160141A1

公开(公告)日：2012-06-28

申请号：US13407785

申请日：2012-02-29

Applicant: Yuwei WANG ,  Zhenqiang LIU ,  Jing ZHANG ,  Qian YU

Inventor： Yuwei WANG ,  Zhenqiang LIU ,  Jing ZHANG ,  Qian YU

IPC: F23J1/06

CPC classification number: F23J1/02

Abstract: A bottom ash discharging device for a coal-fired boiler including: a clinker case, installed between a bottom ash hopper outlet of the coal-fired boiler and a bottom ash conveyer, an impact bar including a plurality of heat-resistant metal rods supporting inside the clinker case, a clinker crusher, installed above the impact bar for crushing the clinker, and a guiding device, installed between the bottom ash hopper outlet and the clinker crusher for limiting the clinker to drop into the clinker crushing area of the clinker crusher. The bottom ash discharging device ensures that the clinkers drop from the bottom ash hopper outlet to the rear area of the clinker crusher, and that the impact bar can be replaced and repaired without stopping the coal-fired boiler.

Abstract translation: 一种用于燃煤锅炉的底灰排放装置，包括：安装在燃煤锅炉的底灰料斗出口和底灰输送机之间的熟料箱，包括多个支撑在内部的耐热金属棒的冲击杆 熟料盒，熟料破碎机，安装在冲击棒上方，用于破碎熟料;以及引导装置，安装在底灰斗出料口和熟料破碎机之间，用于限制熟料落入熟料破碎机的熟料破碎区域。 底灰排放装置可确保熟料从底灰料斗出口落到熟料破碎机的后部区域，并可在不停止燃煤锅炉的情况下更换和修理冲击棒。

公开(公告)号：US20170273283A1

公开(公告)日：2017-09-28

申请号：US15083269

申请日：2016-03-28

Applicant: Rui HOU ,  Yuwei WANG ,  Huifang LIANG ,  Zhanguo ZHANG ,  Zhimin LIU ,  Binhao ZHANG ,  Bixiang ZHANG ,  Xiaoping CHEN

Inventor： Rui HOU ,  Yuwei WANG ,  Huifang LIANG ,  Zhanguo ZHANG ,  Zhimin LIU ,  Binhao ZHANG ,  Bixiang ZHANG ,  Xiaoping CHEN

IPC: A01K67/027 ,  C12N9/02 ,  C12N5/09

CPC classification number: A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  C12N5/0693 ,  C12N9/0069 ,  C12Y113/12007

Abstract: A method for establishing an animal model of hepatocellular carcinoma (HCC) bone metastasis, the method including: 1) establishing 97H and LM3 cell clones with stable expression of firefly luciferase (LUC); 2) allowing HCC cells to form bone metastasis in nude mice via intratibial injection; 3) reproducing HCC bone metastasis in nude mice via intracardiac injection of tumor cells; and 4) isolating a sub-population of tumor cells that targets metastasis to bone. The 97H and the LM3 are highly metastatic HCC cell lines transfected with luciferase gene. BALB/cA-nu mice are 4-5 weeks old and maintained in laminar flow cabinets under SPF conditions and received human care throughout an entire study. A cell number for intratibial injection is 0.5×106, and a cell number for intracardiac injection is 1×106.

公开(公告)号：USD678581S1

公开(公告)日：2013-03-19

申请号：US29413227

申请日：2012-02-13

Applicant: Jifang Du ,  Wei Wang ,  Jinhua Zhang ,  Yuwei Wang

Designer： Jifang Du ,  Wei Wang ,  Jinhua Zhang ,  Yuwei Wang

公开(公告)号：USD674527S1

公开(公告)日：2013-01-15

申请号：US29413153

申请日：2012-02-11

Applicant: Jifang Du ,  Wei Wang ,  Jinhua Zhang ,  Yuwei Wang

Designer： Jifang Du ,  Wei Wang ,  Jinhua Zhang ,  Yuwei Wang