公开(公告)号：US09807986B2

公开(公告)日：2017-11-07

申请号：US15083269

申请日：2016-03-28

申请人： Rui Hou ,  Yuwei Wang ,  Huifang Liang ,  Zhanguo Zhang ,  Zhimin Liu ,  Binhao Zhang ,  Bixiang Zhang ,  Xiaoping Chen

发明人： Rui Hou ,  Yuwei Wang ,  Huifang Liang ,  Zhanguo Zhang ,  Zhimin Liu ,  Binhao Zhang ,  Bixiang Zhang ,  Xiaoping Chen

IPC分类号： A01K67/027 ,  A61K49/00 ,  G01N33/50 ,  C12N5/09 ,  C12N9/02

CPC分类号： A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  C12N5/0693 ,  C12N9/0069 ,  C12Y113/12007

摘要： A method for establishing an animal model of hepatocellular carcinoma (HCC) bone metastasis, the method including: 1) establishing 97H and LM3 cell clones with stable expression of firefly luciferase (LUC); 2) allowing HCC cells to form bone metastasis in nude mice via intratibial injection; 3) reproducing HCC bone metastasis in nude mice via intracardiac injection of tumor cells; and 4) isolating a sub-population of tumor cells that targets metastasis to bone. The 97H and the LM3 are highly metastatic HCC cell lines transfected with luciferase gene. BALB/cA-nu mice are 4-5 weeks old and maintained in laminar flow cabinets under SPF conditions and received human care throughout an entire study. A cell number for intratibial injection is 0.5×106, and a cell number for intracardiac injection is 1×106.