公开(公告)号：US20240200042A1

公开(公告)日：2024-06-20

申请号：US18598120

申请日：2024-03-07

申请人： ZHENHE PHARMACEUTICAL (HANGZHOU) CO., LTD.

发明人： GANG WANG ,  YIN YU ,  JIAN HUANG ,  HUALIN YI

IPC分类号： C12N9/12 ,  A61P9/00 ,  C07K14/005 ,  C07K14/435 ,  C07K14/47 ,  C07K14/55 ,  C12N9/22

CPC分类号： C12N9/127 ,  A61P9/00 ,  C07K14/005 ,  C07K14/435 ,  C07K14/43595 ,  C07K14/4715 ,  C07K14/55 ,  C12N9/22 ,  C12Y207/07048 ,  C12N2710/12022 ,  C12N2710/16622 ,  C12N2710/20022 ,  C12N2740/16022 ,  C12N2760/16122 ,  C12N2770/20022

摘要： The application relates to the technical field of biomedicine, in particular to a limited self-replicating mRNA molecular system, producing method and use. The limited self-replicating mRNA molecular system including: a first mRNA encoding a mutated alphavirus replicase; and at least one second mRNA encoding a target protein; by generating specific mutation adjustments in the nsP2 subunit of mutated replicase, this limited self-replicating mRNA molecular system can achieve limited self-replication and avoid cytotoxicity; by constructing different mRNA with mutated alphavirus replicase and different target proteins, the mutated alphavirus replicase encoded by the first mRNA can simultaneously replicate multiple different target proteins, achieving sustained expression of multiple target proteins.