公开(公告)号：US20060270012A1

公开(公告)日：2006-11-30

申请号：US11365275

申请日：2006-03-01

申请人： Nigel Bowers ,  Paul Skonezny ,  Gregory Stein ,  Thomas Franceschini ,  Shu-Jen Chiang ,  Wendy Anderson ,  Li You ,  Zizhou Xing

发明人： Nigel Bowers ,  Paul Skonezny ,  Gregory Stein ,  Thomas Franceschini ,  Shu-Jen Chiang ,  Wendy Anderson ,  Li You ,  Zizhou Xing

IPC分类号： C12P7/02

CPC分类号： C12R1/01 ,  C12P13/001

摘要： The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648.