公开(公告)号：US20200248176A1

公开(公告)日：2020-08-06

申请号：US16841411

申请日：2020-04-06

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila Belhocine ,  Geoffrey MCDERMOTT ,  Francesca MESCHI ,  Xinying ZHENG

IPC: C12N15/10

Abstract: Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US20230340565A1

公开(公告)日：2023-10-26

申请号：US18097989

申请日：2023-01-17

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila BELHOCINE ,  Josephine Lee ,  Francesca MESCHI ,  Luz MONTESCLAROS ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Jerald SAPIDA ,  David SUKOVICH ,  Tobias Daniel WHEELER ,  Yifeng YIN

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.