公开(公告)号：US20220236258A1

公开(公告)日：2022-07-28

申请号：US17716134

申请日：2022-04-08

Applicant: 10x Genomics, Inc.

Inventor： Geoffrey MCDERMOTT ,  Sarah TAYLOR ,  Stephane Claude BOUTET ,  Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON

IPC: G01N33/543

Abstract: Provided herein are methods and systems for measuring secreted cytokines or other analytes from single cells. The methods disclosed herein include the use of analyte-specific and/or barcoded binding agents (e.g., antibodies) and beads in partitions (e.g., droplets or wells) to measure such analytes on a single cell basis. Further described herein are methods comprising the use of hydrogel-encapsulated cells (e.g., cell beads) to measure secreted and/or cellular analytes from single cells.

公开(公告)号：US20230383283A1

公开(公告)日：2023-11-30

申请号：US18297137

申请日：2023-04-07

Applicant: 10x Genomics, Inc.

Inventor： Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON ,  Geoffrey MCDERMOTT

IPC: C12N15/10

CPC classification number: C12N15/1037 ,  C12N15/1065

Abstract: Provided herein are methods and systems for measuring secreted antibodies or other analytes from single cells. The methods disclosed herein include the use of capture agents and reporter agents that bind to secreted antibodies, wherein the capture agent and/or the reporter agent can be analyte-specific and/or barcoded, and beads in partitions (e.g., droplets or wells) to measure secreted antibodies on a single cell basis. Further described herein are methods comprising the use of hydrogel-encapsulated cells (e.g., cell beads) to measure secreted and/or cellular analytes from single cells.

公开(公告)号：US20220228201A1

公开(公告)日：2022-07-21

申请号：US17565028

申请日：2021-12-29

Applicant: 10x Genomics, Inc.

Inventor： Geoffrey MCDERMOTT ,  David Michael PATTERSON ,  Andrew D. PRICE ,  Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN

IPC: C12Q1/6837 ,  C12Q1/6869

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, provided herein is photocontrollable hybridization, where oligonucleotides with photo-caged bases prevent hybridization of complementary nucleic acid strands. Uncaging of the oligonucleotides allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

公开(公告)号：US20250075272A1

公开(公告)日：2025-03-06

申请号：US18951533

申请日：2024-11-18

Applicant: 10x Genomics, Inc.

Inventor： Solongo B. ZIRALDO ,  Geoffrey MCDERMOTT ,  Shea Thompson LANCE

IPC: C12Q1/6883 ,  C12Q1/6827 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

Abstract: The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

公开(公告)号：US20220113318A1

公开(公告)日：2022-04-14

申请号：US17497135

申请日：2021-10-08

Applicant: 10x Genomics, Inc. ,  10x Genomics Ltd

Inventor： Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON ,  Geoffrey MCDERMOTT

IPC: G01N33/68 ,  G01N33/532

Abstract: The present disclosure relates in some aspects to methods and compositions for assessing the target binding capacity of a binding molecule, such as an antigen binding molecule, e.g., a monoclonal antibody. In some embodiments, provided herein is a method of analyzing binding of a barcode-labeled antibody to a target antigen expressed by a cell, wherein cells expressing the candidate antigens are partitioned and wherein detection of the barcoded nucleic acid molecule is indicative of the binding between the antibody or antigen-binding fragment thereof and the candidate antigen or epitope of the cell.

公开(公告)号：US20200248176A1

公开(公告)日：2020-08-06

申请号：US16841411

申请日：2020-04-06

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila Belhocine ,  Geoffrey MCDERMOTT ,  Francesca MESCHI ,  Xinying ZHENG

IPC: C12N15/10

Abstract: Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.