公开(公告)号：US20210190770A1

公开(公告)日：2021-06-24

申请号：US17131174

申请日：2020-12-22

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Jill HERSCHLEB ,  Adam LOWE ,  Albert KIM ,  Meiliana TJANDRA

IPC: G01N33/53 ,  C12N9/48 ,  C07D239/24 ,  C07D213/04

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.