公开(公告)号：US20230304072A1

公开(公告)日：2023-09-28

申请号：US18187981

申请日：2023-03-22

Applicant: 10x Genomics, Inc.

Inventor： Shalini GOHIL ,  Shea Thompson LANCE ,  Geoffrey P. MCDERMOTT ,  David Michael PATTERSON ,  Andrew D. PRICE

IPC: C12Q1/6806 ,  C12N15/10 ,  C08J3/075 ,  B01J13/02

CPC classification number: C12Q1/6806 ,  B01J13/02 ,  C08J3/075 ,  C12N15/1082 ,  C08J2333/26

Abstract: The present disclosure relates generally to compositions and methods for generating hydrogel coatings on features. In some embodiments, hydrogel-coated features are prepared by horseradish peroxidase-mediated gelation of microspheres. Some embodiments relate to methods for generating hydrogel coatings using particle templated emulsification. The disclosure also relates to the use of hydrogel-coated features in arrays for spatial analysis of biological analytes in biological samples.

公开(公告)号：US20240167081A1

公开(公告)日：2024-05-23

申请号：US18503949

申请日：2023-11-07

Applicant: 10x Genomics,Inc.

Inventor： Felice Alessio BAVA ,  Cheyenne CHRISTOPHERSON ,  Justin COSTA ,  Shalini GOHIL ,  Christina GALONSKA ,  Monica NAGENDRAN

IPC: C12Q1/682 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N1/30

CPC classification number: C12Q1/682 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N1/30

Abstract: The present disclosure relates in some aspects to methods and compositions for immobilization of nucleic acids for in situ detection in a biological sample or a matrix embedding the biological sample.

公开(公告)号：US20240150816A1

公开(公告)日：2024-05-09

申请号：US18478248

申请日：2023-09-29

Applicant: 10x Genomics, Inc.

Inventor： Hongbo FENG ,  Shalini GOHIL ,  Malte KÜHNEMUND

IPC: C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6844

CPC classification number: C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6844

Abstract: The present disclosure in some aspects relates to methods and compositions for processing a biological sample, including methods and compositions for targeted shrinking and expansion of biomolecules in a biological sample for in situ analysis. In some aspects, a polymer is conjugated with a probe or product thereof (e.g., rolling circle amplification products) in the biological sample, and the polymer conjugate may be shrunk or expanded. In some aspects, a size-controllable polymer network is used to shrink or expand the biomolecules in the biological sample. Targeted shrinking can create more space for analyzing more biomolecules at the same time inside a confined space within the sample and expand the space between adjacent probes or products thereof at locations in the sample.

公开(公告)号：US20240351041A1

公开(公告)日：2024-10-24

申请号：US18634751

申请日：2024-04-12

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON ,  Justin COSTA ,  Shalini GOHIL ,  Hong CHEN ,  Yufeng QIAN ,  Minkyung PARK ,  Xiaoyang CAI ,  Hiroshi SASAKI ,  Naveed BAKH

IPC: B01L3/00

CPC classification number: B01L3/527 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/12

Abstract: The present disclosure provides systems and methods for reagent storage. System may include one or more substrates comprising a partition. The partition may be configured to retain a first reagent separate from a second reagent. The partition may include a seal disposed between the first and second reagent. The seal may be configured to maintain the first reagent separate from the second reagent. The first and second reagent may be disposed in a first layer and a second layer, respectively, of frozen material. The first layer and second layer of frozen material may maintain the first reagent separate from the second reagent.

公开(公告)号：US20230295556A1

公开(公告)日：2023-09-21

申请号：US18188164

申请日：2023-03-22

Applicant: 10x Genomics, Inc.

Inventor： Shalini GOHIL ,  Joshua DELANEY ,  Yi LUO ,  Adam LOWE ,  Jason C. BELL ,  Michael John Terry STUBBINGTON ,  Wyatt James MCDONNELL

IPC: C12M1/00 ,  B01L3/02 ,  B01L3/00

CPC classification number: C12M47/04 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0652

Abstract: The present disclosure provides methods, compositions, and systems for selective enzymatic gelation of cells, e.g., immune cells, contained in partitions.

公开(公告)号：US20190233878A1

公开(公告)日：2019-08-01

申请号：US16374112

申请日：2019-04-03

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/00 ,  B01J13/18

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20240271183A1

公开(公告)日：2024-08-15

申请号：US18542404

申请日：2023-12-15

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/00 ,  B01J13/18 ,  C08J3/075 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  B01J13/0065 ,  B01J13/18 ,  C08J3/075 ,  C08J2333/00 ,  C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20220364150A1

公开(公告)日：2022-11-17

申请号：US17861503

申请日：2022-07-11

Applicant: 10x Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C08J3/075

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20210190770A1

公开(公告)日：2021-06-24

申请号：US17131174

申请日：2020-12-22

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Jill HERSCHLEB ,  Adam LOWE ,  Albert KIM ,  Meiliana TJANDRA

IPC: G01N33/53 ,  C12N9/48 ,  C07D239/24 ,  C07D213/04

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.