公开(公告)号：US20240401109A1

公开(公告)日：2024-12-05

申请号：US18745320

申请日：2024-06-17

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Nabil Mikhaiel ,  Augusto Manuel Tentori ,  David Michael Patterson ,  Yifeng Yin ,  Sultan Doganay Tuncer ,  Meghan L. F. Frey ,  Shea Thompson Lance

IPC: C12Q1/6806 ,  C12Q1/6841

Abstract: The present disclosure features methods, compositions, and kits for multiple barcoding, high-density barcoding, and/or selective release of barcoded capture probes to capture analytes, or proxies thereof, from a biological sample.

公开(公告)号：US20250051847A1

公开(公告)日：2025-02-13

申请号：US18929514

申请日：2024-10-28

Applicant: 10x Genomics, Inc.

Inventor： Solongo B. ZIRALDO ,  Geoffrey McDermott ,  Shea Thompson Lance

IPC: C12Q1/6883 ,  C12Q1/6827 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

Abstract: The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

公开(公告)号：US12275993B2

公开(公告)日：2025-04-15

申请号：US18929514

申请日：2024-10-28

Applicant: 10x Genomics, Inc.

Inventor： Solongo B. Ziraldo ,  Geoffrey McDermott ,  Shea Thompson Lance

IPC: C12Q1/6883 ,  C12Q1/6827 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

Abstract: The present disclosure relates to methods, compositions and systems for droplet processing. For example, methods can include (a) providing a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules; (b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent; and (c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.

公开(公告)号：US20220025438A1

公开(公告)日：2022-01-27

申请号：US17397775

申请日：2021-08-09

Applicant: 10X Genomics, Inc.

Inventor： Solongo B. Ziraldo ,  Geoffrey McDermott ,  Shea Thompson Lance

IPC: C12Q1/6806 ,  C12Q1/6804

Abstract: The disclosure provides systems and methods for droplet processing. For example, a method can include providing a first droplet and a second droplet. In some cases, the first droplet may have a first concentration of a reagent and the second droplet may have a second concentration of the reagent. The second droplet may comprise a bead or a biological particle. The method can also include subjecting the first droplet and the second droplet to conditions sufficient to transfer the reagent from the first droplet to the second droplet, thereby decreasing the first concentration in the first droplet and increasing the second concentration in the second droplet.