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    Methods, compositions, and kits for blocking a capture probe on a spatial array
    5.
    发明授权
    Methods, compositions, and kits for blocking a capture probe on a spatial array 有权

    公开(公告)号：US11753673B2

    公开(公告)日：2023-09-12

    申请号：US18181263

    申请日：2023-03-09

    Applicant: 10x Genomics, Inc.

    Inventor： Jennifer Chew ,  David Michael Patterson

    IPC: C12Q1/68 ,  C12Q1/6806

    CPC classification number: C12Q1/6806

    Abstract: Provided are methods for determining a location of a target nucleic acid in a biological sample including: disposing the biological sample onto an array including a plurality of capture probes, where a first capture probe includes a first spatial barcode and a capture domain and a second capture probe includes a second spatial barcode and the capture domain. The second capture probe is not covered by the biological sample on the array and is contacted with a solution comprising TdT and one or more dideoxynucleotides, such that a dideoxynucleotide is incorporated into the second capture domain. Target nucleic acids are captured by the first capture probe, and the sequence of the first spatial barcode or a complement thereof and all or a portion of a sequence of the target nucleic acid, or a complement thereof, are used to determine the location of the target nucleic acid in the biological sample.

    Methods, compositions, and kits for blocking a capture probe on a spatial array
    9.
    发明授权
    Methods, compositions, and kits for blocking a capture probe on a spatial array 有权

    公开(公告)号：US11840724B2

    公开(公告)日：2023-12-12

    申请号：US18226933

    申请日：2023-07-27

    Applicant: 10x Genomics, Inc.

    Inventor： Jennifer Chew ,  David Michael Patterson

    IPC: C12Q1/68 ,  C12Q1/6806

    CPC classification number: C12Q1/6806

    Abstract: Provided herein are methods for determining a location of a protein in a biological sample including: disposing the sample onto an array comprising a plurality of capture probes, where a first and second capture probe include a first and second spatial barcode, respectively, and a capture domain. The second capture probe is not covered by the sample and is contacted with a solution comprising TdT and one or more dideoxynucleotides, where a dideoxynucleotide is incorporated into the second capture domain. Analyte capture agents comprising an analyte binding moiety that binds protein and an oligonucleotide including an analyte capture sequence and an analyte binding moiety barcode are contacted with the sample. The analyte capture sequence hybridizes to the first capture domain, and the sequence of the first spatial barcode and the analyte binding moiety barcode, or complements thereof, are used to determine the location of the protein in the biological sample.

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