公开(公告)号：US20210230681A1

公开(公告)日：2021-07-29

申请号：US17155764

申请日：2021-01-22

Applicant: 10x Genomics, Inc.

Inventor： David Michael Patterson ,  Sultan Doganay Tuncer ,  Zachary Bent

IPC: C12Q1/6837 ,  C12N9/12 ,  C12N9/00

Abstract: The present disclosure provides methods and compositions for detecting and spatially locating analyte interactions and gene expression in a biological sample. For example, provided herein are methods of determining a location of at least one analyte in a biological sample using analyte-binding moieties, proximity ligation, and an array including capture probes.

公开(公告)号：US11827935B1

公开(公告)日：2023-11-28

申请号：US17531093

申请日：2021-11-19

Applicant: 10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Yifeng Yin ,  Sultan Doganay Tuncer

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6862

CPC classification number: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6862

Abstract: Provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample using splint oligonucleotides, circularized second strands, and rolling circle amplification. For example, provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample where a splint oligonucleotide hybridizes to a second strand; the second strand is ligated together thereby creating a circularized second strand, rolling circle amplification of the circularized second strand results in generation of an amplified second strand, and all or part of the sequence of the amplified second strand is determined and used to spatially detect the analyte in the biological sample.

公开(公告)号：US12297486B2

公开(公告)日：2025-05-13

申请号：US17991309

申请日：2022-11-21

Applicant: 10x Genomics, Inc.

Inventor： David Michael Patterson ,  Sultan Doganay Tuncer ,  Zachary Bent

IPC: C12Q1/68 ,  C12N9/00 ,  C12N9/12 ,  C12Q1/6813 ,  C12Q1/6837 ,  C12Q1/6844

Abstract: The present disclosure provides methods and compositions for detecting and spatially locating analyte interactions and gene expression in a biological sample. For example, provided herein are methods of determining a location of at least one analyte in a biological sample using analyte-binding moieties, proximity ligation, and an array including capture probes.

公开(公告)号：US20230077364A1

公开(公告)日：2023-03-16

申请号：US17991309

申请日：2022-11-21

Applicant: 10x Genomics, Inc.

Inventor： David Michael Patterson ,  Sultan Doganay Tuncer ,  Zachary Bent

IPC: C12Q1/6837 ,  C12N9/12 ,  C12N9/00 ,  C12Q1/6813 ,  C12Q1/6844

Abstract: The present disclosure provides methods and compositions for detecting and spatially locating analyte interactions and gene expression in a biological sample. For example, provided herein are methods of determining a location of at least one analyte in a biological sample using analyte-binding moieties, proximity ligation, and an array including capture probes.

公开(公告)号：US20240401109A1

公开(公告)日：2024-12-05

申请号：US18745320

申请日：2024-06-17

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Nabil Mikhaiel ,  Augusto Manuel Tentori ,  David Michael Patterson ,  Yifeng Yin ,  Sultan Doganay Tuncer ,  Meghan L. F. Frey ,  Shea Thompson Lance

IPC: C12Q1/6806 ,  C12Q1/6841

Abstract: The present disclosure features methods, compositions, and kits for multiple barcoding, high-density barcoding, and/or selective release of barcoded capture probes to capture analytes, or proxies thereof, from a biological sample.

公开(公告)号：US20240084383A1

公开(公告)日：2024-03-14

申请号：US18511481

申请日：2023-11-16

Applicant: 10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Yifeng Yin ,  Sultan Doganay Tuncer

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6862

CPC classification number: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6862

Abstract: Provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample using splint oligonucleotides, circularized second strands, and rolling circle amplification. For example, provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample where a splint oligonucleotide hybridizes to a second strand; the second strand is ligated together thereby creating a circularized second strand, rolling circle amplification of the circularized second strand results in generation of an amplified second strand, and all or part of the sequence of the amplified second strand is determined and used to spatially detect the analyte in the biological sample.