公开(公告)号：US20240363194A1

公开(公告)日：2024-10-31

申请号：US18764600

申请日：2024-07-05

申请人： A-Alpha Bio

发明人： Ryan Emerson ,  Randolph Lopez ,  David Younger

IPC分类号： G16B15/30 ,  C12N15/10 ,  G16B35/20

CPC分类号： G16B15/30 ,  C12N15/1037 ,  G16B35/20

摘要： The present disclosure provides methods for improving libraries for screening protein-protein interactions (PPI) by excluding amino acid substitutions that are predicted to have a redundant or minimal effect on binding between protein binding partners.

公开(公告)号：US11466265B2

公开(公告)日：2022-10-11

申请号：US17516237

申请日：2021-11-01

申请人： A-Alpha Bio, Inc.

发明人： David Younger ,  Randolph Lopez ,  Arpita Sen ,  Ryan Emerson

IPC分类号： C12N15/10 ,  C12N1/16

摘要： Provided herein are methods for identifying pairs of protein binding partners, mutations of which may inform the discovery of pharmaceutically useful small molecules. The methods disclosed herein may allow for the adaptation of the native protein degradation system to modulate specific disease targets at the protein level, in particular, for targets that have long been considered undruggable.

公开(公告)号：US20220090052A1

公开(公告)日：2022-03-24

申请号：US17516237

申请日：2021-11-01

申请人： A-Alpha Bio, Inc.

发明人： David Younger ,  Randolph Lopez ,  Arpita Sen ,  Ryan Emerson

IPC分类号： C12N15/10 ,  C12N1/16

摘要： Provided herein are methods for identifying pairs of protein binding partners, mutations of which may inform the discovery of pharmaceutically useful small molecules. The methods disclosed herein may allow for the adaptation of the native protein degradation system to modulate specific disease targets at the protein level, in particular, for targets that have long been considered undruggable.

公开(公告)号：US20240360439A1

公开(公告)日：2024-10-31

申请号：US18291791

申请日：2022-09-09

申请人： A-Alpha Bio, Inc.

发明人： David Younger ,  Randolph Lopez ,  Ryan Emerson

IPC分类号： C12N15/10

CPC分类号： C12N15/1068

摘要： Provided are methods for resolving the relationship between unique user-designed and/or random synthetic DNA barcodes and a protein-coding mutated region of interest with enhanced accuracy that is amenable to short-read sequencing platforms. In addition, the methods introduce increased sequence divergence between mutational variants of a region of interest in order to enhance the resolvability of mutational variants within a mutagenic library when error-prone long-read sequencing platforms are used.

公开(公告)号：US20240309358A1

公开(公告)日：2024-09-19

申请号：US18423075

申请日：2024-01-25

申请人： A-Alpha Bio, Inc.

发明人： David Younger ,  Randolph Lopez ,  Ryan Emerson

IPC分类号： C12N15/10

CPC分类号： C12N15/1065

摘要： Provided are methods for resolving the relationship between unique user-designed and/or random synthetic DNA barcodes and a protein-coding mutated region of interest with enhanced accuracy that is amenable to short-read sequencing platforms. In addition, the methods introduce increased sequence divergence between mutational variants of a region of interest in order to enhance the resolvability of mutational variants within a mutagenic library when error-prone long-read sequencing platforms are used.