公开(公告)号：US09890414B2

公开(公告)日：2018-02-13

申请号：US14092645

申请日：2013-11-27

申请人： Abwiz Bio, Inc.

发明人： Toshiaki Maruyama ,  Shumpei Maruyama ,  Shigeru CJ Okumura

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/191

摘要： This disclosure relates to methods for creating engineered templates that are useful for amplification of one or more antibody genes without the use of gene-specific primers. More specifically, templates engineered using these methods in a polymerase chain reaction setting which allows for the specific amplification of one or more antibody genes.

公开(公告)号：US20240352445A1

公开(公告)日：2024-10-24

申请号：US18683502

申请日：2022-08-15

申请人： ABWIZ BIO, INC.

发明人： Toshiaki MARUYAMA ,  Shigeru CJ Okumura ,  Kevin ENTZMINGER

IPC分类号： C12N15/10 ,  C07K16/10 ,  G01N33/68

CPC分类号： C12N15/1037 ,  C07K16/1003 ,  C12N15/1058 ,  G01N33/6845

摘要： The present disclosure discloses a method for identifying an optimized protein. The method involves constructing and targeting targeted libraries against a target antigen using a first set of selection conditions to select a pool of binders in each library, combining the selected libraries into one or more libraries; and selecting the combined library against the target antigen using a second set of selection conditions to identify at least one protein having an optimized functional profile. An exemplar protein that can be identified with this method is an antibody or a fragment thereof.

公开(公告)号：US20140162321A1

公开(公告)日：2014-06-12

申请号：US14092645

申请日：2013-11-27

申请人： Abwiz Bio, Inc.

发明人： Toshiaki Maruyama ,  Shumpei Maruyama ,  Shigeru CJ Okumura

IPC分类号： C12P19/34

CPC分类号： C12Q1/6811 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/191

摘要： This disclosure relates to methods for creating engineered templates that are useful for amplification of one or more antibody genes without the use of gene-specific primers. More specifically, templates engineered using these methods in a polymerase chain reaction setting which allows for the specific amplification of one or more antibody genes.

摘要翻译： 本公开涉及用于产生可用于扩增一种或多种抗体基因而不使用基因特异性引物的工程模板的方法。 更具体地，在聚合酶链反应设置中使用这些方法工程化的模板，其允许一个或多个抗体基因的特异性扩增。