公开(公告)号：US20200061205A1

公开(公告)日：2020-02-27

申请号：US16111250

申请日：2018-08-24

Applicant: ACADEMIA SINICA ,  KAOHSIUNG MEDICAL UNIVERSITY

Inventor： STEVEN R. ROFFLER ,  TIAN-LU CHENG ,  CHIEN-HAN KAO ,  BING-MAE CHEN ,  YU-CHENG SU ,  HSIN-YI TUNG ,  KUO-HSIANG CHUANG

IPC: A61K47/68 ,  A61P35/00 ,  C07K16/32 ,  A61K39/395 ,  C07K16/28 ,  C07K16/26 ,  A61K47/14 ,  A61K47/69

Abstract: Disclosed herein is a bi-specific antibody that specifically directs a therapeutic agent to a cancer cell by targeting a tumor antigen of the cancer cell, and thereby suppressing the growth of the cancer or blocking the invasion or metastasis of the cancer. The bi-specific antibody of the present disclosure includes a first antigen binding site that binds to polyethylene glycol (PEG); and a second antigen binding site that binds to a target ligand, such as a tumor antigen.

公开(公告)号：US20140038208A1

公开(公告)日：2014-02-06

申请号：US14057999

申请日：2013-10-18

Applicant: KAOHSIUNG MEDICAL UNIVERSITY

Inventor： TIAN-LU CHENG ,  STEVEN R. ROFFLER ,  KUO-HSIANG CHUANG ,  SSU-JUNG LU

IPC: G01N33/554

CPC classification number: G01N33/554 ,  C07K16/44 ,  C07K2317/56 ,  C07K2319/03 ,  C12N2503/02 ,  C12N2510/04 ,  G01N33/5308 ,  G01N33/6857 ,  G01N33/94 ,  G01N33/9493 ,  G01N2430/60 ,  G01N2440/28

Abstract: The present invention provides a method for preparing an isolated eukaryotic cell which presents an anti-polyethylene glycol (PEG) antibody on a cell membrane. The present invention also provides a method for a quantitative analysis of a polyethylene glycol (PEG) by said anti-PEG antibody expressing cell. The cell-based quantitative analysis of the present prevention could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.

Abstract translation: 本发明提供了一种在细胞膜上提供抗聚乙二醇（PEG）抗体的分离的真核细胞的制备方法。 本发明还提供了通过所述抗-PEG抗体表达细胞定量分析聚乙二醇（PEG）的方法。 本发明预防的基于细胞的定量分析可以灵敏地定量游离的PEG和PEG修饰的大分子（蛋白质，纳米颗粒和脂质体）作为纳克级水平敏感。