公开(公告)号：US20200061205A1

公开(公告)日：2020-02-27

申请号：US16111250

申请日：2018-08-24

Applicant: ACADEMIA SINICA ,  KAOHSIUNG MEDICAL UNIVERSITY

Inventor： STEVEN R. ROFFLER ,  TIAN-LU CHENG ,  CHIEN-HAN KAO ,  BING-MAE CHEN ,  YU-CHENG SU ,  HSIN-YI TUNG ,  KUO-HSIANG CHUANG

IPC: A61K47/68 ,  A61P35/00 ,  C07K16/32 ,  A61K39/395 ,  C07K16/28 ,  C07K16/26 ,  A61K47/14 ,  A61K47/69

Abstract: Disclosed herein is a bi-specific antibody that specifically directs a therapeutic agent to a cancer cell by targeting a tumor antigen of the cancer cell, and thereby suppressing the growth of the cancer or blocking the invasion or metastasis of the cancer. The bi-specific antibody of the present disclosure includes a first antigen binding site that binds to polyethylene glycol (PEG); and a second antigen binding site that binds to a target ligand, such as a tumor antigen.

公开(公告)号：US20190022245A1

公开(公告)日：2019-01-24

申请号：US16111242

申请日：2018-08-24

Applicant: ACADEMIA SINICA ,  KAOHSIUNG MEDICAL UNIVERSITY

Inventor： STEVEN R ROFFLER ,  TIAN-LU CHENG ,  CHIEN-HAN KAO ,  BING-MAE CHEN ,  YU-CHENG SU ,  HSIN-YI TUNG ,  Kuo-Hsiang Chuang

IPC: A61K47/68 ,  C07K16/32 ,  A61K49/00 ,  C07K16/28 ,  C07K16/30 ,  C07K16/44

Abstract: Disclosed herein is a bi-specific antibody that specifically directs a therapeutic agent to a cancer cell by targeting a tumor antigen of the cancer cell, and thereby suppressing the growth of the cancer or blocking the invasion or metastasis of the cancer. The bi-specific antibody of the present disclosure includes a first antigen binding site that binds to polyethylene glycol (PEG); and a second antigen binding site that binds to a target ligand, such as a tumor antigen.

公开(公告)号：US20210154319A9

公开(公告)日：2021-05-27

申请号：US16111242

申请日：2018-08-24

Applicant: ACADEMIA SINICA ,  KAOHSIUNG MEDICAL UNIVERSITY

Inventor： STEVEN R ROFFLER ,  TIAN-LU CHENG ,  CHIEN-HAN KAO ,  BING-MAE CHEN ,  YU-CHENG SU ,  HSIN-YI TUNG ,  Kuo-Hsiang Chuang

IPC: A61K47/68 ,  C07K16/32 ,  A61K49/00 ,  C07K16/28 ,  C07K16/30 ,  C07K16/44

Abstract: Disclosed herein is a bi-specific antibody that specifically directs a therapeutic agent to a cancer cell by targeting a tumor antigen of the cancer cell, and thereby suppressing the growth of the cancer or blocking the invasion or metastasis of the cancer. The bi-specific antibody of the present disclosure includes a first antigen binding site that binds to polyethylene glycol (PEG); and a second antigen binding site that binds to a target ligand, such as a tumor antigen.

公开(公告)号：US20170056523A1

公开(公告)日：2017-03-02

申请号：US15123243

申请日：2015-03-02

Applicant: ACADEMIA SINICA ,  KAOHSIUNG MEDICAL UNIVERSITY

Inventor： STEVEN R ROFFLER ,  TIAN-LU CHENG ,  CHIEN-HAN KAO ,  BING-MAE CHEN ,  YU-CHENG SU ,  HSIN-YI TUNG ,  Kuo-Hsiang Chuang

IPC: A61K47/48 ,  A61K49/00 ,  C07K16/28 ,  C07K16/32 ,  C07K16/44 ,  C07K16/30

CPC classification number: A61K47/6879 ,  A61K47/6897 ,  A61K49/0002 ,  C07K16/2803 ,  C07K16/2863 ,  C07K16/2887 ,  C07K16/30 ,  C07K16/3061 ,  C07K16/32 ,  C07K16/44 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/622 ,  C07K2317/624 ,  C07K2317/73

Abstract: Disclosed herein is a bi-specific antibody that specifically directs a therapeutic agent to a cancer cell by targeting a tumor antigen of the cancer cell, and thereby suppresses the growth of the cancer or blocking the invasion or metastasis of the cancer. The bi-specific antibody of the present disclosure includes a first antigen binding site that binds to polyethylene glycol (PEG); and a second antigen binding site that binds to a target ligand, such as a tumor antigen.

Abstract translation: 本文公开了通过靶向癌细胞的肿瘤抗原来特异性地将治疗剂引导至癌细胞的双特异性抗体，从而抑制癌症的生长或阻断癌症的侵袭或转移。 本公开的双特异性抗体包括结合聚乙二醇（PEG）的第一抗原结合位点; 以及结合靶配体例如肿瘤抗原的第二抗原结合位点。

公开(公告)号：US20140038208A1

公开(公告)日：2014-02-06

申请号：US14057999

申请日：2013-10-18

Applicant: KAOHSIUNG MEDICAL UNIVERSITY

Inventor： TIAN-LU CHENG ,  STEVEN R. ROFFLER ,  KUO-HSIANG CHUANG ,  SSU-JUNG LU

IPC: G01N33/554

CPC classification number: G01N33/554 ,  C07K16/44 ,  C07K2317/56 ,  C07K2319/03 ,  C12N2503/02 ,  C12N2510/04 ,  G01N33/5308 ,  G01N33/6857 ,  G01N33/94 ,  G01N33/9493 ,  G01N2430/60 ,  G01N2440/28

Abstract: The present invention provides a method for preparing an isolated eukaryotic cell which presents an anti-polyethylene glycol (PEG) antibody on a cell membrane. The present invention also provides a method for a quantitative analysis of a polyethylene glycol (PEG) by said anti-PEG antibody expressing cell. The cell-based quantitative analysis of the present prevention could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.

Abstract translation: 本发明提供了一种在细胞膜上提供抗聚乙二醇（PEG）抗体的分离的真核细胞的制备方法。 本发明还提供了通过所述抗-PEG抗体表达细胞定量分析聚乙二醇（PEG）的方法。 本发明预防的基于细胞的定量分析可以灵敏地定量游离的PEG和PEG修饰的大分子（蛋白质，纳米颗粒和脂质体）作为纳克级水平敏感。

公开(公告)号：US20160356784A1

公开(公告)日：2016-12-08

申请号：US15243975

申请日：2016-08-23

Applicant: KAOHSIUNG MEDICAL UNIVERSITY

Inventor： TIAN-LU CHENG ,  Steve ROFFLER ,  WEN-WEI LIN ,  I-JU CHEN

IPC: G01N33/68 ,  C07K14/00 ,  G01N33/58

CPC classification number: G01N33/6803 ,  C07K14/00 ,  C07K16/00 ,  C07K16/4283 ,  C07K2317/52 ,  C07K2319/00 ,  C07K2319/40 ,  G01N33/58 ,  G01N33/6854 ,  G01N2570/00

Abstract: The present invention is related to a method for detecting a protein comprising: (1) providing a fusion protein, wherein the fusion protein comprises the protein which is fused with a secondary antibody detected protein tag, wherein the secondary antibody detected protein tag comprises at least one epitope selected from the Fc region of at least one primary antibody which is capable of being detected by a corresponding secondary antibody; (2) contacting the fusion protein with a secondary antibody which binds to the secondary antibody detected protein tag to form a protein/antibody complex; and (3) detecting the protein/antibody complex.

Abstract translation: 本发明涉及一种检测蛋白质的方法，包括：（1）提供融合蛋白，其中所述融合蛋白包含与第二抗体检测到的蛋白质标签融合的蛋白质，其中所述第二抗体检测到的蛋白质标签至少包含 选自能够被相应二级抗体检测的至少一种一抗的Fc区的一个表位; （2）使融合蛋白与二次抗体接触，二次抗体与二次抗体检测的蛋白质标签结合以形成蛋白质/抗体复合物; 和（3）检测蛋白/抗体复合物。

公开(公告)号：US20150315265A1

公开(公告)日：2015-11-05

申请号：US14534174

申请日：2014-11-06

Applicant: KAOHSIUNG MEDICAL UNIVERSITY

Inventor： TIAN-LU CHENG ,  Steve ROFFLER ,  WEN-WEI LIN ,  I-JU CHEN

IPC: C07K16/00

CPC classification number: G01N33/6803 ,  C07K14/00 ,  C07K16/00 ,  C07K16/4283 ,  C07K2317/52 ,  C07K2319/00 ,  C07K2319/40 ,  G01N33/58 ,  G01N33/6854 ,  G01N2570/00

Abstract: A method of preparing an epitope for a secondary antibody detected protein tag, comprising: constructing a expressing vector, comprising a nucleotide sequence for encoding the secondary antibody detected protein tag comprising at least one epitope selected from at least one primary antibody which is detected by corresponding secondary antibody; expressing the vector in a host cell; and obtaining the secondary antibody detected protein tag which is expressed in the host cell. Further, a method for constructing a protein molecular weight marker ladder, comprising constructing a plurality of recombinant protein tags with different molecular weights, wherein each the recombinant protein tag comprises at least one epitope of primary antibody. Besides, a secondary antibody detected protein tag comprises at least two peptide sequences of epitopes selected from primary antibodies of at least two different species.

Abstract translation: 一种制备二抗检测蛋白质标签的表位的方法，包括：构建表达载体，其包含用于编码第二抗体检测的蛋白质标签的核苷酸序列，所述核苷酸序列包含至少一个选自至少一个第一抗体的表位，所述表位被相应的 二抗; 在宿主细胞中表达载体; 并获得在宿主细胞中表达的二抗检测蛋白质标签。 此外，构建蛋白质分子量标记梯的方法，包括构建多个具有不同分子量的重组蛋白质标签，其中每个重组蛋白质标签包含一抗的至少一个表位。 此外，二次抗体检测的蛋白质标签包含至少两个选自至少两种不同物种的一抗的表位的肽序列。