公开(公告)号：US20230338501A1

公开(公告)日：2023-10-26

申请号：US18024703

申请日：2021-07-04

Applicant: ACCESS TO ADVANCED HEALTH INSTITUTE

Inventor： Neal VAN HOEVEN ,  Emily VOIGT

IPC: A61K39/12 ,  C12N7/00 ,  A61P31/14 ,  A61K9/107

CPC classification number: A61K39/12 ,  C12N7/00 ,  A61P31/14 ,  A61K9/1075 ,  A61K2039/53

Abstract: This disclosure provides ribonucleic acid (RNA) polynucleotides encoding replication-competent viral genomes that, when introduced to a subject, induce an active viral replication. The RNA may be provided naked or with an artificial RNA delivery system. The viral genome may be a full-length genome of an attenuated viral strain. For example, the RNA may encode an attenuated Chikungunya or yellow fever virus. The artificial RNA delivery system may be a lipid particle such as a lipid nanoparticle (LNP), a nanostructure lipid carrier (NLC), or a cationic nanoemulsion (CNE). This disclosure also provides methods of inducing an immune response, including protective immunity, by administering to a subject an RNA polynucleotide that encodes a replication-competent viral genome in an amount sufficient to cause viral replication in the subject. The immune response may include inducing the production of neutralizing antibodies at a level comparable to inoculation with a live-attenuated virus.