公开(公告)号：US10774312B2

公开(公告)日：2020-09-15

申请号：US15573877

申请日：2016-05-02

申请人： ADVANCED CELL TECHNOLOGY AND ENGINEERING LTD.

发明人： Toshimitsu Higa ,  Kenji Otani ,  Koichi Otomo

IPC分类号： C12N5/0775 ,  A61K35/28 ,  A61K35/32 ,  A61L27/00 ,  A61L27/38 ,  A61L27/54 ,  A61L27/58 ,  C12N5/077

摘要： It is an object of the present invention to provide a method for producing frozen mesenchymal cells maintaining a viability and an adhesion rate similar to those of cells obtained through a step of culture, without undergoing a step of culturing cells before cryopreservation step. The method comprises dissociating cells by treating a tissue with proteolytic enzymes and cryopreserving the cells. The present invention provides a method for producing frozen mesenchymal cells maintaining a viability and an adhesion rate similar to those of culturing cells without a step of culturings cells before cryopreserving.