公开(公告)号：US20170266653A1

公开(公告)日：2017-09-21

申请号：US15614944

申请日：2017-06-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. POLLACK ,  Ramakrishna SISTA ,  Zhishan HUA ,  Vamsee K. PAMULA ,  Arjun SUDARSAN ,  Vijay SRINIVASAN ,  Prasanna THWAR ,  Alexander SHENDEROV ,  Dwayne ALLEN

IPC: B01L3/02 ,  B03C1/28 ,  B01F13/08 ,  B01L3/00 ,  B01F13/00 ,  B03C1/01

CPC classification number: B01F13/0023 ,  B01F13/002 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0818 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0631 ,  B01L2200/0657 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/043 ,  B01L2400/0478 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/284 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  Y10T436/25

Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.