公开(公告)号：US20160002290A1

公开(公告)日：2016-01-07

申请号：US14769245

申请日：2014-02-20

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley GAGNON ,  Vincent VAGENENDE

IPC: C07K1/36 ,  C07K1/34 ,  C07K1/30 ,  C07K1/18

CPC classification number: C07K1/36 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/18 ,  C07K1/30 ,  C07K1/34

Abstract: A method includes (i) adding allantoin in a supersaturating amount to a protein preparation including a desired protein and at least one endotoxin as a contaminant, (ii) removing solids after the adding step to provide a sample for further purification by void exclusion chromatography on a packed particle bed of electropositive particles in a column, the packed particle bed having an interparticle volume, (iii) applying a sample volume to the packed particle bed, wherein the electropositive particles support void exclusion chromatography, and wherein the sample volume is not greater than the interparticle volume, and (iv) eluting a purified sample including the desired protein and a reduced amount of the endotoxin. The method is optionally carried out with only the allantoin treatment or only the void exclusion chromatography.

Abstract translation: 一种方法包括（i）将超滤量的尿囊素加入到包含所需蛋白质和至少一种作为污染物的内毒素的蛋白质制剂中，（ii）在加入步骤后除去固体，以提供样品进一步通过空隙排阻色谱法进行纯化 在柱中的正电性颗粒的填充颗粒床，所述填充颗粒床具有颗粒间体积，（iii）将样品体积施加到所述填充颗粒床，其中所述正电荷颗粒支持空隙排阻层析，并且其中所述样品体积不大 （iv）洗脱含有所需蛋白质和减少量内毒素的纯化样品。 该方法任选仅通过尿囊素处理或仅进行空白排阻色谱进行。