公开(公告)号：US12173340B2

公开(公告)日：2024-12-24

申请号：US16461320

申请日：2017-11-16

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Pao Chun Lin ,  Zhiwei Song

IPC: C12N15/52 ,  C12N9/00 ,  C12N15/09 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.

公开(公告)号：US20240124868A1

公开(公告)日：2024-04-18

申请号：US18493395

申请日：2023-10-24

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Pao Chun Lin ,  Zhiwei Song

IPC: C12N9/00 ,  C12N15/52 ,  C12N15/85

CPC classification number: C12N9/93 ,  C12N15/52 ,  C12N15/85 ,  C12N2840/203 ,  C12Y603/01002

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.

公开(公告)号：US20250136966A1

公开(公告)日：2025-05-01

申请号：US18946537

申请日：2024-11-13

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Pao Chun Lin ,  Zhiwei Song

IPC: C12N9/00 ,  C12N15/52 ,  C12N15/85

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.

公开(公告)号：US20190352631A1

公开(公告)日：2019-11-21

申请号：US16461320

申请日：2017-11-16

Applicant: AGENCY FOR SCIENCE TECHNOLOGY AND RESEARCH

Inventor： Pao Chun Lin ,  Zhiwei Song

IPC: C12N9/00 ,  C12N15/52 ,  C12N15/85

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.