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公开(公告)号：US20250136966A1
公开(公告)日：2025-05-01
申请号：US18946537
申请日：2024-11-13
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Pao Chun Lin , Zhiwei Song
Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.
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公开(公告)号：US20190352631A1
公开(公告)日：2019-11-21
申请号：US16461320
申请日：2017-11-16
Applicant: AGENCY FOR SCIENCE TECHNOLOGY AND RESEARCH
Inventor： Pao Chun Lin , Zhiwei Song
Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.
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公开(公告)号：US09273292B2
公开(公告)日：2016-03-01
申请号：US14636795
申请日：2015-03-03
Inventor： Zhiwei Song
IPC: C12N15/00 , C12P21/00 , C12N9/10 , C07K14/505 , C07K14/57
CPC classification number: C12N9/1051 , C07K14/505 , C07K14/57 , C12N2510/02 , C12P21/005 , C12Y204/0115
Abstract: We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).
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4.发明申请OPTIMISED HEAVY CHAIN AND LIGHT CHAIN SIGNAL PEPTIDES FOR THE PRODUCTION OF RECOMBINANT ANTIBODY THERAPEUTICS 审中-公开用于生产重组抗体治疗的优化重链和轻链信号肽公开(公告)号：US20150225482A1
公开(公告)日：2015-08-13
申请号：US14435094
申请日：2013-08-21
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Zhiwei Song
CPC classification number: C07K16/2887 , C07K16/22 , C07K16/241 , C07K16/2863 , C07K16/3015 , C07K16/3061 , C07K16/32 , C07K2317/24 , C07K2319/02 , C12N15/635
Abstract: Antibodies of interest comprising a heavy chain signal peptide and/or a light chain signal peptide or combination thereof and compositions are described. The method of determining the signal peptide including creating data set of antibodies signal peptides, clustering, selecting and creating recombinant antibodies for enhanced expression and secretion are described.
Abstract translation: 描述了包含重链信号肽和/或轻链信号肽或其组合的组合物所关注的抗体。 描述了确定信号肽的方法，包括产生抗体信号肽的数据集，聚集，选择和产生用于增强表达和分泌的重组抗体。
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公开(公告)号：US20250011825A1
公开(公告)日：2025-01-09
申请号：US18711011
申请日：2022-12-20
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Zhiwei Song , Wen Min Lau
Abstract: This technology relates to the production of lactose and human milk oligosaccharides (HMOs) in cells.
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Patent Agency Ranking
6.发明授权公开(公告)号：US09243235B2
公开(公告)日：2016-01-26
申请号：US14620856
申请日：2015-02-12
Applicant: Agency for Science, Technology and Research
Inventor： Zhiwei Song
CPC classification number: C12N9/2445 , C12N9/2402 , C12P21/005 , C12Y204/01101 , C12Y302/01045
Abstract: We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-I into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.
Abstract translation: 我们描述了从宿主细胞中表达包含甘露糖封端的N-聚糖的重组蛋白的方法，所述方法包括：（a）将编码重组蛋白的核酸引入中国仓鼠卵巢（CHO）细胞中，所述中国仓鼠卵巢（CHO）细胞包含 GnT 1基因（GenBank登录号AF343963）导致GnT1功能丧失; 和（c）从宿主细胞中表达重组蛋白，其中表达的重组蛋白包含甘露糖封端的聚糖结构，并且其中该方法不包括将功能性GnT-I引入宿主细胞的步骤。 该方法可用于生产具有甘露糖封端的聚糖结构的重组葡糖脑苷脂酶，适用于治疗或预防戈谢氏病。
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公开(公告)号：US20150184135A1
公开(公告)日：2015-07-02
申请号：US14636795
申请日：2015-03-03
Inventor： Zhiwei Song
IPC: C12N9/10 , C07K14/505 , C12P21/00
CPC classification number: C12N9/1051 , C07K14/505 , C07K14/57 , C12N2510/02 , C12P21/005 , C12Y204/0115
Abstract: We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).
Abstract translation: 我们提供一种与野生型中国仓鼠卵巢细胞相比能够更高蛋白质唾液酸化的中国仓鼠卵巢（CHO）细胞，例如在功能性GnT 1存在下，其中CHO细胞可通过用蓖麻凝集素选择获得 我（RCA-I）。
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公开(公告)号：US20150119558A1
公开(公告)日：2015-04-30
申请号：US14373182
申请日：2013-01-18
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Zhiwei Song
IPC: C07K14/47
CPC classification number: C07K14/4727 , A61K39/0011 , A61K2039/505 , C07K14/705 , C12N9/1051 , C12N2510/02 , C12P21/005
Abstract: The present invention provides modified cells for producing proteins with modified glycosylation patterns. Proteins produced in such cells, and the use of such proteins in medicine, and particularly in the treatment of cancer, is also provided.
Abstract translation: 本发明提供用于产生具有修饰的糖基化模式的蛋白质的修饰细胞。 还提供了在这种细胞中产生的蛋白质，以及这些蛋白质在医药中的用途，特别是在治疗癌症中。
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公开(公告)号：US12173340B2
公开(公告)日：2024-12-24
申请号：US16461320
申请日：2017-11-16
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Pao Chun Lin , Zhiwei Song
Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.
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公开(公告)号：US20240124868A1
公开(公告)日：2024-04-18
申请号：US18493395
申请日：2023-10-24
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Pao Chun Lin , Zhiwei Song
CPC classification number: C12N9/93 , C12N15/52 , C12N15/85 , C12N2840/203 , C12Y603/01002
Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.
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