公开(公告)号：US11982606B2

公开(公告)日：2024-05-14

申请号：US16803503

申请日：2020-02-27

Applicant: Agilent Technologies, Inc.

Inventor： Victor Lim ,  Carol T. Schembri ,  Adrienne Mccampbell ,  Nazumi Alice Yama-Dang

IPC: G01N1/44 ,  G01N1/30 ,  G01N1/31 ,  G01N1/36 ,  G01N1/40

CPC classification number: G01N1/44 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  G01N1/36 ,  G01N1/40 ,  G01N2001/366 ,  G01N2001/4038

Abstract: Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.

公开(公告)号：US20240272051A1

公开(公告)日：2024-08-15

申请号：US18632030

申请日：2024-04-10

Applicant: Agilent Technologies, Inc.

Inventor： Victor Lim ,  Carol T. Schembri ,  Adrienne Mccampbell ,  Nazumi Alice Yama-Dang

IPC: G01N1/44 ,  G01N1/30 ,  G01N1/31 ,  G01N1/36 ,  G01N1/40

CPC classification number: G01N1/44 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  G01N1/36 ,  G01N1/40 ,  G01N2001/366 ,  G01N2001/4038

Abstract: Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.

公开(公告)号：US20180120207A1

公开(公告)日：2018-05-03

申请号：US15797232

申请日：2017-10-30

Applicant: Agilent Technologies, Inc.

Inventor： Victor Lim ,  Carol T. Schembri ,  Adrienne Mccampbell ,  Nazumi Alice Yama-Dang

IPC: G01N1/44 ,  G01N1/40 ,  G01N1/30

CPC classification number: G01N1/44 ,  G01N1/30 ,  G01N1/312 ,  G01N1/36 ,  G01N1/40 ,  G01N2001/366 ,  G01N2001/4038

Abstract: Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.

公开(公告)号：US10550425B2

公开(公告)日：2020-02-04

申请号：US15351312

申请日：2016-11-14

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Nicholas M. Sampas ,  Nazumi Alice Yama-Dang ,  Paige Anderson

IPC: C12Q1/68 ,  C12Q1/6832

Abstract: Provided herein, among other things, are compositions for improving signal-to-noise in hybridization to oligonucleotide arrays. In some embodiments, the composition may comprise a population of indexing oligonucleotides of formula X-Y, where the sequences of region X hybridize to an array and the sequences of region Y hybridize to distinct sites within a genomic region, transcript, set of transcripts, or complement of the same. Use of these oligonucleotides increases signal, reduces background and smoothens array hybridization analyses. Methods and kits that employ the compositions are also provided.

公开(公告)号：US20180135115A1

公开(公告)日：2018-05-17

申请号：US15351312

申请日：2016-11-14

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Nicholas M. Sampas ,  Nazumi Alice Yama-Dang ,  Paige Anderson

IPC: C12Q1/68

CPC classification number: C12Q1/6832 ,  C12Q1/6837 ,  C12Q2565/514

Abstract: Provided herein, among other things, are compositions for improving signal-to-noise in hybridization to oligonucleotide arrays. In some embodiments, the composition may comprise a population of indexing oligonucleotides of formula X-Y, where the sequences of region X hybridize to an array and the sequences of region Y hybridize to distinct sites within a genomic region, transcript, set of transcripts, or complement of the same. Use of these oligonucleotides increases signal, reduces background and smoothens array hybridization analyses. Methods and kits that employ the compositions are also provided.