公开(公告)号：US20240272051A1

公开(公告)日：2024-08-15

申请号：US18632030

申请日：2024-04-10

Applicant: Agilent Technologies, Inc.

Inventor： Victor Lim ,  Carol T. Schembri ,  Adrienne Mccampbell ,  Nazumi Alice Yama-Dang

IPC: G01N1/44 ,  G01N1/30 ,  G01N1/31 ,  G01N1/36 ,  G01N1/40

CPC classification number: G01N1/44 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  G01N1/36 ,  G01N1/40 ,  G01N2001/366 ,  G01N2001/4038

Abstract: Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.

公开(公告)号：US20180348105A1

公开(公告)日：2018-12-06

申请号：US15775391

申请日：2016-12-02

Applicant: SOUTHWEST PETROLEUM UNIVERSITY

Inventor： Ping GUO ,  Shuai WU ,  Wanbo ZHANG ,  Yisheng HU ,  Yijian CHEN ,  Huimin ZHANG ,  Zhouhua WANG ,  Jianfen DU ,  Huang LIU ,  Hongmei REN

IPC: G01N1/36 ,  G01N15/08

CPC classification number: G01N1/36 ,  G01N15/082 ,  G01N15/088 ,  G01N33/241 ,  G01N2001/2873 ,  G01N2001/366

Abstract: A direct method for manufacturing a large model fractured core and maintaining original oil-water saturation, including the following steps: (1) determining the volume V, porosity φ, permeability K, oil saturation So, water saturation Sw and the like of a fractured core to be manufactured; (2) preparing simulated oil, and determining the used oil mass mo=Vo×ρo; (3) under the circumstance of no consideration of oil saturation, acquiring the mass of the used water, cement and quartz sand; (4) while establishing oil saturation, acquiring the mass mw of water for manufacturing the core as mw=a−Vo×ρw; (5) mixing oil, water and an emulsifier evenly to prepare an oil-in-water emulsion; (6) adding cement and quartz sand into the emulsion and stirring evenly to obtain cement slurry; (7) when a cement sample is in a semi-solidified state, cutting the cement sample with a steel wire; and (8) solidifying the cement sample to the end.

公开(公告)号：US10054523B2

公开(公告)日：2018-08-21

申请号：US14759229

申请日：2013-11-27

Applicant: Sun Mi Jung

Inventor： Sun Mi Jung

IPC: G01N1/36

CPC classification number: G01N1/36 ,  G01N2001/366

Abstract: Disclosed is an embedding cassette for biopsy to embed tissue received in an embedding mold provided with a space to receive the tissue when the embedding cassette is combined with the embedding mold, including a body provided with a space communicating with the receipt space of the combined embedding mold so that an injected paraffin solution may coagulate therein during embedding of the tissue and at least one paraffin barrier groove formed at the edge of the lower surface of the body, and, when the embedding cassette is combined with the embedding mold and embedding of the tissue is carried out, the injected paraffin solution flows into the at least one paraffin barrier groove and forms a barrier and, thereby, leakage of the paraffin solution between the embedding cassette and embedding mold is prevented.

公开(公告)号：US09810611B2

公开(公告)日：2017-11-07

申请号：US14494022

申请日：2014-09-23

Applicant: Cellpath Ltd

Inventor： Paul Webber ,  Philip Webber ,  Richard Titcombe

IPC: A61B10/00 ,  G01N1/31 ,  G01N1/36

CPC classification number: G01N1/312 ,  G01N1/36 ,  G01N2001/366

Abstract: A method of avoiding deformation and providing for a more consistent thickness of a biological tissue sample during preparation for analysis comprising providing a histology processing cassette comprising a box defining a compartment for holding a biological tissue sample, the box having a bottom face comprising a sample support surface and being transmissible to radiation or a flow of fluid through to pass through the cassette, an open top face, and two side walls, a back wall and a front wall, the box having a length and width greater than a standard size histology processing cassette and a depth approximately the same as a standard size histology processing cassette placing the sample in the cassette and treating the sample by contacting it with a processing solution; and contacting the sample with molten paraffin wax and cooling the sample in molten paraffin wax so the sample is embedded in solidified paraffin wax for further processing.

公开(公告)号：US09671322B2

公开(公告)日：2017-06-06

申请号：US14552946

申请日：2014-11-25

Applicant: Leica Biosystems Nussloch GmbH

Inventor： Markus Berberich

IPC: G01N1/36

CPC classification number: G01N1/36 ,  G01N2001/366

Abstract: An automated embedding machine includes at least a conveyor, a pouring station, and a cooling station. The conveyor is embodied and arranged to receive casting molds each having at least one histological sample arranged therein, and to transport them to the pouring station which fills each casting mold with an embedding medium heated to above its melting point, and then to the cooling station which cools the embedding medium of each casting mold to below its melting point. The disclosure furthermore relates to a method for embedding a histological sample.

公开(公告)号：US20150087018A1

公开(公告)日：2015-03-26

申请号：US14493985

申请日：2014-09-23

Applicant: CellPath Ltd.

Inventor： Paul Webber ,  Philip Webber ,  Richard Titcombe

IPC: G01N1/31 ,  G01N35/00

CPC classification number: G01N35/00732 ,  G01N1/36 ,  G01N2001/366 ,  G01N2035/00801 ,  G01N2035/00831

Abstract: A histology processing cassette comprising a box comprising a compartment for holding a biological tissue sample, the box having a bottom face comprising at least in part a sample support surface and being transmissible to radiation, an open top face, and two side walls, a back wall and a front wall, the box having dimensions greater than a standard size histology processing cassette and comprising a recess in the front wall adapted to receive a standard size cassette which has a front wall comprising a unique identifier for the biological tissue sample such that upon insertion of the standard cassette in the recess the unique identifier on the front wall of the standard cassette is readable.

Abstract translation: 一种组织学处理盒，包括一个盒子，该盒子包括用于容纳生物组织样本的隔室，该盒子具有一个底面，该底面至少部分地包括一个样品支撑表面，并且可透射到辐射，一个敞开的顶面和两个侧壁， 壁和前壁，该盒具有大于标准尺寸的组织学处理盒的尺寸，并且包括在前壁中的适于接收标准尺寸盒的凹部，其具有包括用于生物组织样本的唯一标识符的前壁， 将标准盒插入凹槽中，标识盒前壁上的唯一标识符是可读的。

公开(公告)号：US20150008616A1

公开(公告)日：2015-01-08

申请号：US14377854

申请日：2013-12-17

Applicant: UC-CARE LTD.

Inventor： Alex Pasternak ,  Keren Schweizer

IPC: G01N1/36 ,  B29B13/00

CPC classification number: G01N1/36 ,  B29B13/00 ,  B29C43/18 ,  G01N2001/366

Abstract: A mold for producing a biological tissue embedded in a block of an embedding material is provided. The mold comprises a compartment having a compartment floor and a depression extending downwards from the compartment floor. A molding apparatus, comprising a mold and a press for pressing a sample sheet onto the compartment floor of the mold is provided. The press comprises a foot configured to enter at least partially into the compartment and press a sample tissue, at least partially into the depression. A method of embedding a biological tissue in an embedding material using a mold as described herein is provided. A cleaning device configured for removing excess embedding materials from the press of the molding apparatus is provided.

Abstract translation: 提供一种用于生产嵌入嵌入材料块中的生物组织的模具。 模具包括具有隔室底板和从隔室底板向下延伸的凹部的隔间。 提供了一种模制设备，其包括用于将样品片材压到模具的隔室底板上的模具和压力机。 压力机包括构造成至少部分地进入隔室并将样品组织至少部分地压入凹陷中的脚。 提供了使用如本文所述的模具将生物组织嵌入嵌入材料的方法。 提供了一种清洁装置，其被构造成用于从模制设备的压机中去除多余的嵌入材料。

公开(公告)号：US20140273084A1

公开(公告)日：2014-09-18

申请号：US14204587

申请日：2014-03-11

Applicant: LEICA BIOSYSTEMS NUSSLOCH GMBH

Inventor： Florian BOEHL ,  Stella KNORR ,  Peter KOTLARSKI ,  Graeme ROBERTSON ,  Chris RYAN ,  Ian SOHN ,  Wilfred BAUWENS ,  Fernando DIAS

IPC: G01N1/31

CPC classification number: G01N1/31 ,  B01L3/508 ,  B01L2300/0609 ,  G01N1/36 ,  G01N2001/315 ,  G01N2001/366

Abstract: An apparatus for holding a tissue sample having a retaining member with a first tissue engaging surface and at least one biasing element. The first tissue engaging surface is moveably attached to the retaining member. The apparatus also has a base comprising a second tissue engaging surface which is configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other. The apparatus also has a retracting member connected to the retaining member which is configured to retract the first tissue engaging surface and compress the biasing element to form a gap between the tissue sample and one of the first tissue engaging surface and the second tissue engaging surface.

Abstract translation: 一种用于保持具有第一组织接合表面的保持构件的组织样本和至少一个偏置元件的装置。 第一组织接合表面可移动地附接到保持构件。 该装置还具有包括第二组织接合表面的底座，该第二组织接合表面构造成与保持构件接合以形成第一和第二组织接合表面彼此面对的内部区域。 该装置还具有连接到保持构件的缩回构件，该缩回构件被构造成缩回第一组织接合表面并且压缩偏置元件以在组织样本与第一组织接合表面和第二组织接合表面中的一个之间形成间隙。

公开(公告)号：US20140271407A1

公开(公告)日：2014-09-18

申请号：US14204375

申请日：2014-03-11

Applicant: LEICA BIOSYSTEMS NUSSLOCH GMBH

Inventor： Stella KNORR ,  Andrew GUY ,  Ralf ECKERT ,  Fiona TARBET ,  Fernando DIAS ,  Chris RYAN ,  Neil SANUT

IPC: G01N1/31

CPC classification number: G01N1/31 ,  B01L3/508 ,  B01L3/5457 ,  B01L2200/14 ,  B01L2300/021 ,  B01L2300/043 ,  B01L2300/0609 ,  B01L2300/12 ,  B01L2300/123 ,  G01N1/36 ,  G01N2001/315 ,  G01N2001/366

Abstract: An apparatus for holding a tissue sample including a retaining member having a first tissue engaging surface and at least one biasing element, the first tissue engaging surface being moveably attached to the retaining member by said biasing element; and a base having a second tissue engaging surface and configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other, wherein the at least one biasing element urges the first tissue engaging surface toward the second tissue engaging surface to retain the tissue sample therebetween in the interior area.

Abstract translation: 一种用于保持组织样本的装置，包括具有第一组织接合表面和至少一个偏置元件的保持构件，所述第一组织接合表面通过所述偏置元件可移动地附接到所述保持构件; 以及具有第二组织接合表面并且构造成与所述保持构件接合以形成所述第一和第二组织接合表面彼此面对的内部区域的底座，其中所述至少一个偏压元件将所述第一组织接合表面推向所述第二组织 接合表面以将组织样本保持在内部区域中。

公开(公告)号：US08734735B2

公开(公告)日：2014-05-27

申请号：US13047044

申请日：2011-03-14

Applicant: Warren P. Williamson, IV ,  Thomas J. Ward ,  Stephen P. Whitlatch

Inventor： Warren P. Williamson, IV ,  Thomas J. Ward ,  Stephen P. Whitlatch

IPC: A61B10/00

CPC classification number: G01N1/36 ,  G01N35/00732 ,  G01N2001/366 ,  Y10T436/2525 ,  Y10T436/2575

Abstract: An automated machine for handling and embedding tissue samples contained on microtome sectionable supports. The machine includes an input member configured to hold a plurality of the microtome sectionable supports prior to a tissue embedding operation. An output member is configured to hold a plurality of the microtome sectionable supports after the tissue embedding operation. A cooling unit is configured to hold at least one of the microtome sectionable supports during the tissue embedding operation. A motorized carrier assembly is mounted for movement and configured to hold at least one of the microtome sectionable supports. The carrier assembly moves the support from the input member to the cooling unit and, finally, to the output member. A dispensing device dispenses an embedding material onto the microtome sectionable support and at least one tissue sample carried by the microtome sectionable support during the embedding operation.

Abstract translation: 一种用于处理和嵌入包含在切片机可切片支架上的组织样本的自动化机器。 该机器包括一个输入构件，构造成在组织嵌入操作之前保持多个切片机可切割支架。 输出构件被配置为在组织嵌入操作之后保持多个切片机可切割支撑件。 冷却单元构造成在组织嵌入操作期间保持切片机可分段支撑件中的至少一个。 安装电动载体组件用于移动并且构造成保持切片机可分段支撑件中的至少一个。 托架组件将支撑件从输入构件移动到冷却单元，最后移动到输出构件。 分配装置在嵌入操作期间将嵌入材料分配到切片机可切片支撑件和由切片机可切割支撑件携带的至少一个组织样本。