公开(公告)号：US20170044513A1

公开(公告)日：2017-02-16

申请号：US15252664

申请日：2016-08-31

Applicant: AJINOMOTO CO., INC.

Inventor： Noriko YOKOYAMA ,  Mayu NAKANO ,  Shuhei HASHIRO ,  Masako HIRAGA ,  Kazutaka SHIMBO ,  Ayaka TOKUNAGA

IPC: C12N9/82 ,  A21D2/26 ,  A23C9/12 ,  A23L13/40 ,  A23L27/60 ,  C12P21/00 ,  A23C13/16

Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.

Abstract translation: 一种新型蛋白质脱酰胺酶，其具有直接作用于蛋白质中的天冬酰胺残基的侧链酰胺基的活性，形成侧链羧基并释放氨，产生该氨基酸的微生物，编码该氨基酸的基因， 生产它们，并提供其使用。 培养分类为放线杆菌的细菌以产生蛋白质脱酰胺酶，并从培养物中收集酶。