公开(公告)号：US20240218015A1

公开(公告)日：2024-07-04

申请号：US18598901

申请日：2024-03-07

Applicant: Ajinomoto Co., Inc.

Inventor： Yutaka MATSUDA ,  Yuichi NAKAHARA ,  Kei YAMADA ,  Keisuke KATO ,  Yuta ENDO ,  Brian Alan MENDELSOHN

IPC: C07K1/04 ,  C07K1/02

CPC classification number: C07K1/045 ,  C07K1/02

Abstract: A method for producing an intermediate antibody regioselectively modified, which comprises:



(1) mixing a solution comprising an antibody material and a solution comprising a reagent for regioselectively modifying an antibody in a micromixer to form a mixture comprising the antibody material and the reagent,
wherein the reagent comprises a compound comprising an affinity substance to an antibody and a reactive group to an antibody; and
(2) passing the mixture through a reaction channel to allow the antibody material and the reagent to react in the reaction channel to form a solution comprising an intermediate antibody regioselectively modified,
wherein the processes (1) and (2) are continuously performed in a flow microreactor,
is capable of rapidly producing a desired antibody derivative regioselectively having a functional substance or functional substances.

公开(公告)号：US20210253629A1

公开(公告)日：2021-08-19

申请号：US17308594

申请日：2021-05-05

Applicant: AJINOMOTO CO., INC.

Inventor： Daisuke EJIMA ,  Yuichi NAKAHARA ,  Keisuke KATO ,  Masayo DATE

IPC: C07K1/113 ,  B01J19/24

Abstract: The present invention provides a method for producing a refolded protein, the method comprising a step for mixing, inside a micro mixer, a buffer and a solubilization solution for a protein that has lost higher order structure or that has become insoluble.