公开(公告)号：US20220348884A1

公开(公告)日：2022-11-03

申请号：US17863832

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Yusuke OHYA ,  Megumi NISHIYAMA ,  Atsushi KONISHI

IPC: C12N5/074

Abstract: A medium with a reduced osmotic pressure containing a basal medium, in which the osmotic pressure is adjusted to 150 to 250 mOsm/kg, is useful for culturing cells.

公开(公告)号：US20210147794A1

公开(公告)日：2021-05-20

申请号：US17159573

申请日：2021-01-27

Applicant: AJINOMOTO CO., INC.

Inventor： Kenichiro ITO ,  Shumpei FUROMITSU ,  Yusuke OHYA ,  Takuya HIGUCHI

IPC: C12N5/00

Abstract: Additives and media containing a water-soluble polymer are useful for suspension culture of animal cells, and can suppress the precipitation of medium components such as insulin 5 and the like due to physical stimulation of stirring, shaking, circulation, gas bubbling, or the like in suspension culture of animal cells, and can improve the culture efficiency of animal cells and the quality of cultured cells.

公开(公告)号：US20230012723A1

公开(公告)日：2023-01-19

申请号：US17863646

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Megumi NISHIYAMA ,  Kotoe KOSEKI

IPC: C12N5/00 ,  C12N5/074

Abstract: Cells may be cultured by a method including the following steps: a first step of preparing a population of cell aggregates having a major axis of not more than 400 μm, and a second step of suspension culturing the population of cell aggregates obtained in the first step.

公开(公告)号：US20220348883A1

公开(公告)日：2022-11-03

申请号：US17863785

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Megumi NISHIYAMA

IPC: C12N5/074 ,  C12N5/0735

Abstract: A method including adding choline at a specific concentration to a medium is useful for culturing cells.

公开(公告)号：US20230002729A1

公开(公告)日：2023-01-05

申请号：US17863546

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Kenichiro ITO ,  Megumi NISHIYAMA

IPC: C12N5/0735

Abstract: A medium composition, containing a basic fibroblast growth factor (bFGF) at not less than 150 ng/mL is useful for culturing cells.

公开(公告)号：US20210284971A1

公开(公告)日：2021-09-16

申请号：US17213839

申请日：2021-03-26

Applicant: AJINOMOTO CO., INC.

Inventor： Takuya MATSUMOTO ,  Shimpei OGAWA ,  Shumpei FUROMITSU ,  Yusuke OHYA ,  Kotoe KOSEKI ,  Takuya HIGUCHI ,  Megumi NISHIYAMA

IPC: C12N5/074 ,  C12N5/0735

Abstract: By using an additive for culturing animal cells that contains an amino acid or glucose, or an amino acid and glucose; or a medium for culturing animal cells that contains an amino acid or glucose, or an amino acid and glucose in addition to a medium component; or a method for culturing animal cells that includes culturing the animal cells using the aforementioned medium, or adding the aforementioned additive to a culture, the proliferation ability of animal cells can be improved particularly when the animal cells are cultured using a serum-free medium or a low albumin medium.