公开(公告)号：US20180148728A1

公开(公告)日：2018-05-31

申请号：US15540611

申请日：2015-12-31

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Pyung Cheon LEE ,  Bo Hyun CHOI ,  Sun Hyoung CHOI

IPC: C12N15/70 ,  C12P23/00 ,  C12N15/01 ,  C12N9/10 ,  C12N9/02 ,  C12N9/90

CPC classification number: C12N15/70 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1048 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/01 ,  C12P23/00 ,  C12Y103/9903 ,  C12Y114/13129 ,  C12Y205/01001 ,  C12Y205/01032 ,  C12Y505/01019

Abstract: Provided is a mutant having an ability to overproduce carotenoids and a method for producing carotenoids by using the mutant. The mutant, of which mutations are induced by irradiation after being transformed with a recombinant vector according to the subject matter, has an excellent ability to produce carotenoids and can be mass-produced, and thus can be useful in various industrial fields, which use carotenoids, such as cosmetics, food, and feed.

公开(公告)号：US20180371501A1

公开(公告)日：2018-12-27

申请号：US15736931

申请日：2016-06-15

Applicant: Ajou University Industry-Academic Cooperation Foundation

Inventor： Pyung Cheon LEE ,  Jin Ho KIM

IPC: C12P5/00 ,  C12N15/70 ,  C07K14/415 ,  C12N9/10 ,  C12N9/90 ,  C12N15/113

CPC classification number: C12P5/00 ,  C07K14/415 ,  C12N9/1025 ,  C12N9/90 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  C12Y205/01031 ,  C12Y503/03002

Abstract: A method for producing natural rubber by using a recombinant microorganism is disclosed, the method comprising: (a) manufacturing an expression vector capable of expressing a gene encoding a cis-prenyltransferase, which is a guayule-derived natural rubber synthetase represented by the amino acid sequence of SEQ ID NO: 2 or a Hevea brasiliensis-derived natural rubber synthetase represented by the amino acid sequence of SEQ ID NO: 6, and an expression vector capable of expressing a gene coding for a natural rubber precursor synthetase; (b) transforming a host microorganism with the expression vector; (c) culturing the transformed host microorganism; and (d) separating natural rubber from the cultured transformed host microorganism. The natural rubber obtained by the method has a white powder form identical to that of natural rubber, and shows an FT-IR spectrum pattern extremely similar to that of natural rubber.