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公开(公告)号：US20240228986A1
公开(公告)日：2024-07-11
申请号：US18560883
申请日：2022-05-13
申请人： Cellibre, Inc.
发明人： Spiros Kambourakis , Nicholas Donald Keul , Russell Scott Komor , Jun Urano , Nicky Christopher Caiazza
CPC分类号： C12N9/1085 , C12P7/22 , C12Y205/01001 , C07K2319/00
摘要： Disclosed herein are novel CBGA and CBGVA synthases and methods for improvement of their overall activities for the synthesis of CBGA and CBGVA from their respective precursors, olivetolic acid (OA) or divarinic acid (DVA) and GPP. Also disclosed are fusion proteins to enhance synthesis of CBGA and CBGVA. The methods described herein also increase the titer and the purity of CBGA and CBGVA made by a cell by 1) decreasing the formation of byproducts FCBGA and FCBGVA that are synthesized from the respective prenylation of OA and DVA with FPP, and/or 2) increasing the intracellular availability of OA and DVA.
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公开(公告)号：US20240199704A1
公开(公告)日：2024-06-20
申请号：US18443086
申请日：2024-02-15
发明人： Jerome FOX , Ankur SARKAR , Akarawin HONGDUSIT , Edward KIM
IPC分类号： C07K14/005 , C07K14/47 , C12N1/20
CPC分类号： C07K14/005 , C07K14/47 , C12N1/20 , C07K2319/80 , C12Y205/01001 , C12Y205/0101 , C12Y207/01036 , C12Y207/04002 , C12Y301/01048 , C12Y401/01033 , C12Y402/03017 , C12Y402/03018 , C12Y402/03024 , C12Y402/03056 , C12Y503/03002
摘要： This invention relates to the field of genetic engineering. Specifically, the invention relates to the construction of operons to produce biologically active agents. For example, operons may be constructed to produce agents that control the function of biochemical pathway proteins (e.g., protein phosphatases, kinases and/or proteases). Such agents may include inhibitors and modulators that may be used in studying or controlling phosphatase function associated with abnormalities in a phosphatase pathway or expression level. Fusion proteins, such as light activated protein phosphatases, may be genetically encoded and expressed as photoswitchable phosphatases. Systems are provided for use in controlling phosphatase function within living cells or in identifying small molecule inhibitors/activator/modulator molecules of protein phosphatases associated with cell signaling.
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公开(公告)号：US20230151062A1
公开(公告)日：2023-05-18
申请号：US17816937
申请日：2022-08-02
发明人： Jerome FOX , Ankur SARKAR , Akarawin HONGDUSIT , Edward KIM
IPC分类号： C07K14/005 , C07K14/47 , C12N1/20
CPC分类号： C07K14/005 , C07K14/47 , C12N1/20 , C07K2319/80 , C12Y205/01001 , C12Y205/0101 , C12Y207/01036 , C12Y207/04002 , C12Y301/01048 , C12Y401/01033 , C12Y402/03017 , C12Y402/03018 , C12Y402/03024 , C12Y402/03056 , C12Y503/03002
摘要： This invention relates to the field of genetic engineering. Specifically, the invention relates to the construction of operons to produce biologically active agents. For example, operons may be constructed to produce agents that control the function of biochemical pathway proteins (e.g., protein phosphatases, kinases and/or proteases). Such agents may include inhibitors and modulators that may be used in studying or controlling phosphatase function associated with abnormalities in a phosphatase pathway or expression level. Fusion proteins, such as light activated protein phosphatases, may be genetically encoded and expressed as photoswitchable phosphatases. Systems are provided for use in controlling phosphatase function within living cells or in identifying small molecule inhibitors/activator/modulator molecules of protein phosphatases associated with cell signaling.
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4.发明申请ENHANCED PRODUCTION OF ISOPRENE USING HOST CELLS HAVING DECREASED ISPA ACTIVITY 审中-公开使用具有减少的ISPA活性的宿主细胞增强异丙苯的生产公开(公告)号：US20150218590A1
公开(公告)日：2015-08-06
申请号：US14593860
申请日：2015-01-09
发明人： Joseph C. MCAULIFFE , Rachel E. Muir , Alex T. Nielsen , Caroline M. Peres , Dmitrii V. Vaviline , Derek H. Wells
CPC分类号： C12P5/007 , C12N9/1085 , C12N9/88 , C12Y205/01001 , C12Y402/03027 , Y02P20/52
摘要： This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.
摘要翻译： 本发明涉及能够以高效率利用这种重组微生物生产异戊二烯和异戊二烯生产的重组微生物。 在本发明中，改变了ispA基因的功能活性，以减少重组细胞中产生异戊二烯的重组细胞的产生，或在发酵过程中容易受类异戊二烯积累影响的细胞中。 这种降低的ispA基因功能活性能够增强宿主微生物中异戊二烯的合成。
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公开(公告)号：US20140030785A1
公开(公告)日：2014-01-30
申请号：US13952071
申请日：2013-07-26
申请人： Toivo Kallas , Matthew Nelson , Eric Singsaas
发明人： Toivo Kallas , Matthew Nelson , Eric Singsaas
CPC分类号： C12P5/007 , C12N9/88 , C12N9/90 , C12P5/002 , C12P5/026 , C12Y205/01001 , C12Y402/03015 , C12Y402/03027 , C12Y503/03002 , Y02P20/52
摘要： Methods of isoprenoid production are provided by the present invention. In particular, transgenic Synechococcus sp. PCC 7002 cyanobacteria and methods for producing isoprene and pinene using a host transgenic Synechococcus sp. PCC 7002 cyanobacterium are provided.
摘要翻译： 异戊二烯生产方法由本发明提供。 特别是转基因聚球藻 PCC 7002蓝细菌和使用宿主转基因聚球藻生产异戊二烯和蒎烯的方法。 提供PCC 7002蓝藻。
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公开(公告)号：US20180094286A1
公开(公告)日：2018-04-05
申请号：US15281152
申请日：2016-09-30
CPC分类号： C12Y402/03017 , C12N9/1085 , C12N9/88 , C12P5/007 , C12Y205/01001
摘要： The present invention relates to a nucleic acid construct comprising a nucleic acid molecule encoding a protein involved in the biosynthesis of a terpenoid or a precursor thereof, wherein said nucleic acid molecule is operably linked to a derepressible promoter.
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公开(公告)号：US09580728B2
公开(公告)日：2017-02-28
申请号：US15156557
申请日：2016-05-17
发明人： Toivo Kallas , Matthew Nelson , Eric Singsaas
CPC分类号： C12P5/007 , C12N9/88 , C12N9/90 , C12P5/002 , C12P5/026 , C12Y205/01001 , C12Y402/03015 , C12Y402/03027 , C12Y503/03002 , Y02P20/52
摘要： Methods of isoprenoid production are provided by the present invention. In particular, transgenic Synechococcus sp. PCC 7002 cyanobacteria and methods for producing isoprene and pinene using a host transgenic Synechococcus sp. PCC 7002 cyanobacterium are provided.
摘要翻译： 异戊二烯生产方法由本发明提供。 特别是转基因聚球藻 PCC 7002蓝细菌和使用宿主转基因聚球藻生产异戊二烯和蒎烯的方法。 提供PCC 7002蓝藻。
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公开(公告)号：US20170009260A1
公开(公告)日：2017-01-12
申请号：US15156557
申请日：2016-05-17
发明人： Toivo Kallas , Matthew Nelson , Eric Singsaas
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N9/88 , C12N9/90 , C12P5/002 , C12P5/026 , C12Y205/01001 , C12Y402/03015 , C12Y402/03027 , C12Y503/03002 , Y02P20/52
摘要： Methods of isoprenoid production are provided by the present invention. In particular, transgenic Synechococcus sp. PCC 7002 cyanobacteria and methods for producing isoprene and pinene using a host transgenic Synechococcus sp. PCC 7002 cyanobacterium are provided.
摘要翻译： 异戊二烯生产方法由本发明提供。 特别是转基因聚球藻 PCC 7002蓝细菌和使用宿主转基因聚球藻生产异戊二烯和蒎烯的方法。 提供PCC 7002蓝藻。
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公开(公告)号：US09388431B2
公开(公告)日：2016-07-12
申请号：US14593860
申请日：2015-01-09
发明人： Joseph C. McAuliffe , Rachel E. Muir , Alex T. Nielsen , Caroline M. Peres , Dmitrii V. Vaviline , Derek H. Wells
CPC分类号： C12P5/007 , C12N9/1085 , C12N9/88 , C12Y205/01001 , C12Y402/03027 , Y02P20/52
摘要： This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.
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公开(公告)号：US20150218589A1
公开(公告)日：2015-08-06
申请号：US14429510
申请日：2013-09-19
发明人： Masahiro Furutani , Akihiro Uenishi , Koichiro Iwasa , Yasuyuki Kori , Seiji Takahashi , Takefumi Shimoyama
CPC分类号： C12P5/007 , C12N9/00 , C12N9/1085 , C12N9/88 , C12N15/52 , C12Y205/01001 , C12Y205/01028 , C12Y402/03051
摘要： To provide a series of techniques for obtaining β-phellandrene with high purity and in a large quantity.Provided is a recombinant cell capable of producing β-phellandrene, prepared by introducing at least one nucleic acid selected from the group consisting of a nucleic acid encoding geranyl pyrophosphate (GPP) synthase and a nucleic acid encoding neryl pyrophosphate (NPP) synthase, and a nucleic acid encoding β-phellandrene synthase into a host cell in such a manner that these nucleic acids are expressed in the host cell. Also provided is a method for producing β-phellandrene by culturing the recombinant cell to produce β-phellandrene in the recombinant cell.
摘要翻译： 提供一系列用于获得高纯度和大量生物化学物质的方法。 提供能够产生通过引入选自由编码香叶基焦磷酸（GPP）合酶的核酸和编码橙色素焦磷酸（NPP）合酶）的核酸组成的组中的至少一种的核酸而制备的重组细胞，以及 以这种方式将宿主细胞中的这些核酸表达在宿主细胞中，将编码β-胡萝卜素合酶的核酸编码到宿主细胞中。 还提供了通过培养重组细胞以在重组细胞中产生并生成β-肾上腺素的方法来生产β-肾上腺素。
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