公开(公告)号：US20170086431A1

公开(公告)日：2017-03-30

申请号：US15274201

申请日：2016-09-23

申请人： ALEXION PHARMA LLC

发明人： Marie-Cecile Van De Lavoir ,  Philip A. Leighton

IPC分类号： A01K67/027 ,  C12N15/85 ,  C07K16/00 ,  C12N5/0735

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/21 ,  C12N5/0611 ,  C12N5/0697 ,  C12N15/85 ,  C12N15/8509 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2502/11 ,  C12N2502/14 ,  C12N2506/11 ,  C12N2510/00 ,  C12N2517/02 ,  C12N2830/008 ,  C12N2830/40

摘要： The present invention is long-term cultures of avian PGCs and techniques to produce germline chimeric and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. These germline chimeric birds do not have substantial contributions of PGC-derived phenotypes in somatic cells or tissues. This invention includes compositions comprising long-term cultures of PGCs that can be genetically modified by gene targeting, that can accept large amounts of foreign DNA and that contribute to the germline of recipient embryos.