公开(公告)号：US20250108121A1

公开(公告)日：2025-04-03

申请号：US18708513

申请日：2022-11-08

Applicant: AMGEN INC.

Inventor： Jonathan DIEP

IPC: A61K47/68 ,  C07K14/605 ,  C07K16/28

Abstract: The present invention relates to mammalian cells modified to provide for reduced cleavage of peptides that will be conjugated to polypeptides expressed by the cells. In particular, the invention relates to a method of producing an antibody peptide conjugate, the method comprising: a) expressing the antibody in a mammalian cell wherein the mammalian cell is a cathepsin D knock out cell, and wherein the antibody comprises a cysteine or non-canonical amino acid amino acid substitution at one or more conjugation site(s); b) purifying the antibody; and c) conjugating a peptide to the antibody at the conjugation site(s).

公开(公告)号：US20240174752A1

公开(公告)日：2024-05-30

申请号：US18284590

申请日：2022-04-26

Applicant: AMGEN INC.

Inventor： Li ZHANG ,  Hedieh BARKHORDARIAN ,  Ewelina ZASADZINSKA ,  Jonathan DIEP

IPC: C07K16/28 ,  C07K14/54

CPC classification number: C07K16/2818 ,  C07K14/54 ,  C07K2317/31 ,  C07K2319/30

Abstract: The present invention relates to the field of biopharmaceutical manufacturing. In particular, the invention relates to using temperature as a lever to modulate product quality during upstream operations.