公开(公告)号：US20200071738A1

公开(公告)日：2020-03-05

申请号：US16676340

申请日：2019-11-06

Applicant: Amgen Inc.

Inventor： Sohye KANG ,  Chung HUANG, JR. ,  Hedieh BARKHORDARIAN ,  Pavel BONDARENKO ,  Zhongqi ZHANG

IPC: C12P21/00 ,  C07K16/00 ,  C07K14/52 ,  C12N5/071 ,  C12N1/38

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US20160333385A1

公开(公告)日：2016-11-17

申请号：US15111470

申请日：2014-12-09

Applicant: AMGEN INC.

Inventor： Sohye KANG ,  Chung HUANG, Jr. ,  Hedieh BARKHORDARIAN ,  Pavel BONDARENKO ,  Zhongqi ZHANG

IPC: C12P21/00 ,  C12N5/071 ,  C07K14/52 ,  C07K16/00

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

Abstract translation: 本发明涉及通过调节细胞培养过程中的鸟氨酸代谢来操纵重组糖蛋白的高甘露糖糖型含量的方法。

公开(公告)号：US20240174752A1

公开(公告)日：2024-05-30

申请号：US18284590

申请日：2022-04-26

Applicant: AMGEN INC.

Inventor： Li ZHANG ,  Hedieh BARKHORDARIAN ,  Ewelina ZASADZINSKA ,  Jonathan DIEP

IPC: C07K16/28 ,  C07K14/54

CPC classification number: C07K16/2818 ,  C07K14/54 ,  C07K2317/31 ,  C07K2319/30

Abstract: The present invention relates to the field of biopharmaceutical manufacturing. In particular, the invention relates to using temperature as a lever to modulate product quality during upstream operations.