专利检索 ap:("AMGEN INC.") AND inv:"Namratha SUBHASH" 第 1 页

1.

发明公开
USING FUCOSIDASE TO CONTROL AFUCOSYLATION LEVEL OF GLYCOSYLATED PROTEINS 审中-公开

公开(公告)号：US20240279704A1

公开(公告)日：2024-08-22

申请号：US18567747

申请日：2022-06-06

申请人： AMGEN INC.

发明人： Namratha SUBHASH , Yu Cong CHEN , Kyle Shamus MCELEARNEY , Glen BOLTON

IPC分类号： C12P21/00 , C07K1/22 , C07K1/34 , C07K16/24 , C12N9/24

CPC分类号： C12P21/005 , C07K1/22 , C07K1/34 , C07K16/244 , C12N9/2402 , C07K2317/14 , C07K2317/41 , C07K2317/72 , C12Y302/01051

摘要： Provided herein are methods of obtaining a recombinant glycosylated protein having increased levels of afucosylated glycoforms. In exemplary embodiments, the methods comprise incubating purified recombinant glycosylated protein with a human broad specificity fucosidase and separating the recombinant glycosylated protein from the fucosidase.