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    METHOD FOR REDUCING FUCOSE CONTENTS OF RECOMBINANT PROTEINS
    5.
    发明申请
    METHOD FOR REDUCING FUCOSE CONTENTS OF RECOMBINANT PROTEINS 审中-公开
    减少重组蛋白质内含物的方法

    公开(公告)号：US20100003742A1

    公开(公告)日：2010-01-07

    申请号：US12351239

    申请日：2009-01-09

    申请人： Jung-Seob KIM ,  Jae-Hoon MOON ,  Mee Sook OH ,  Kong Ju LEE ,  Yeup YOON

    发明人： Jung-Seob KIM ,  Jae-Hoon MOON ,  Mee Sook OH ,  Kong Ju LEE ,  Yeup YOON

    IPC分类号： C07K1/14

    CPC分类号： C07K16/00 ,  C07K2317/41 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/05 ,  C12N9/1051 ,  C12Y204/01068 ,  C12Y302/01051

    摘要： The present invention relates to a method for reducing the fucose content of a recombinant protein, which comprises expressing in an animal cell the recombinant protein and FUCA1, an FUCA1 mutant, FUCA2, or a fragment of FUT8 localization domain; or with a fusion protein of a fragment of FUT8 localization domain and a fragment of FUCA1, a FUCA1 mutant or FUCA2. Therefore, the antibody expressed according to the method of the present invention exhibits a reduced fucose content in their Fc regions, which leads to the improvement in the therapeutic effect thereof.

    摘要翻译： 本发明涉及一种降低重组蛋白的岩藻糖含量的方法，其包括在动物细胞中表达重组蛋白和FUCA1，FUCA1突变体，FUCA2或FUT8定位结构域的片段; 或与FUT8定位结构域的片段和FUCA1，FUCA1突变体或FUCA2的片段的融合蛋白。 因此，根据本发明的方法表达的抗体在其Fc区显示出降低的岩藻糖含量，这导致其治疗效果的改善。

    Isolation and composition of novel glycosidases
    6.
    发明授权
    Isolation and composition of novel glycosidases 失效
    新型糖苷酶的分离和组成

    公开(公告)号：US06300113B1

    公开(公告)日：2001-10-09

    申请号：US08560809

    申请日：1995-11-21

    申请人： David Landry

    发明人： David Landry

    IPC分类号： C12N924

    CPC分类号： C12Y302/01051 ,  C12N9/2402 ,  C12Y302/0105 ,  Y10S435/91

    摘要： Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac&bgr;1-X, Gal&agr;1-3R, Gal&agr;1-6R, Gal&bgr;1-3R, Fuc&agr;-2R, Fuc&agr;1-3R, Fuc&agr;1-4R, Man&agr;1-2R, Man&agr;1-3R, Man&agr;1-6R, Man&bgr;1-4R, Xyl&bgr;1-2R, Glc&bgr;1-4R, and Gal&bgr;1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

    摘要翻译： 已经描述了能够切割选择的糖苷键的基本上纯的糖苷酶，其包括从黄单胞菌属和重组糖苷酶分离的糖苷酶。 已经鉴定了GlcNacbeta1-X，Galalpha1-3R，Galalpha1-6R，Galbeta1-3R，Fucalpha-2R，Fucalpha1-3R，Fucalpha1-4R，Manalpha1-2R，Manalpha1-3R，Manalpha1-6R，Manbeta1的分离的酶的底物特异性 -4R，Xylbeta1-2R，Glcbeta1-4R和Galbeta1-4R，其提供了选择性地切割碳水化合物底物中的糖苷键并形成修饰的碳水化合物的改善的能力。

    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    8.
    发明申请
    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
    纤维素和/或致细胞毒素降解大蒜素的酶及其用途

    公开(公告)号：US20150291945A1

    公开(公告)日：2015-10-15

    申请号：US14421774

    申请日：2013-08-15

    申请人： Bangladesh Jute Research Institute

    发明人： Maqsudul Alam ,  Mohammed S. Islam ,  Mohammed M. Hossen ,  Mohammed S. Haque ,  Mohammed M. Alam

    IPC分类号： C12N9/24 ,  C12N9/38 ,  C12N9/88 ,  C12N9/34 ,  C12N9/42 ,  C12N9/26

    CPC分类号： C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2471 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/88 ,  C12Y302/01037 ,  C12Y302/01051 ,  C12Y302/01055 ,  C12Y302/01059 ,  C12Y302/01075 ,  C12Y302/01089 ,  C12Y302/0113 ,  C12Y302/01177

    摘要： The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

    摘要翻译： 本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，葡糖苷酶，α-葡糖苷酶，木聚糖酶， 甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。 也提供了包含和/或由该酶基因的核苷酸序列组成的载体，表达构建体和宿主细胞。 本发明还提供了用于生产酶的方法和用于改变酶的方法，以便改善其期望的特性。 本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。 这些酶也可用于将木质纤维素生物质完全水解成单糖，然后将其发酵成液体燃料和化学原料。

    ENZYMES FOR INHIBITING GROWTH OF BIOFILMS AND DEGRADING SAME
    10.
    发明申请
    ENZYMES FOR INHIBITING GROWTH OF BIOFILMS AND DEGRADING SAME 有权
    抑制生物膜生长和降解酶的酶

    公开(公告)号：US20140134149A1

    公开(公告)日：2014-05-15

    申请号：US14076701

    申请日：2013-11-11

    申请人： C5-6 Technologies, Inc.

    发明人： Phillip J. Brumm

    IPC分类号： A61K38/47 ,  A01N43/40

    CPC分类号： A61K38/47 ,  A01N43/40 ,  A61K9/0075 ,  A61K31/7036 ,  C12Y302/01001 ,  C12Y302/01004 ,  C12Y302/01025 ,  C12Y302/01051 ,  C12Y302/01091 ,  A61K2300/00

    摘要： Enzymes for inhibiting growth of biofilms and degrading biofilms. The enzymes comprise glycosyl hydrolases capable of degrading biofilms. The enzymes are formulated in compositions with and without antimicrobial agents. The enzymes with and without the antimicrobial agents are delivered to biofilms to degrade the biofilms and treat infections of microorganisms associated with the biofilms, delivered to surfaces to inhibit growth of biofilms thereon, and administered to animals to inhibit growth of biofilms therein.

    摘要翻译： 用于抑制生物膜生长和降解生物膜的酶。 酶包含能够降解生物膜的糖基水解酶。 将酶配制在具有和不具有抗微生物剂的组合物中。 具有和不具有抗微生物剂的酶被递送到生物膜以降解生物膜并治疗与生物膜相关的微生物的感染，被递送到表面以抑制其上的生物膜的生长，并施用于动物以抑制其中生物膜的生长。