公开(公告)号：US12110498B2

公开(公告)日：2024-10-08

申请号：US17690429

申请日：2022-03-09

Applicant: AMYRIS, INC.

Inventor： Kevin George ,  Andrew Main ,  Chia-Hong Tsai

IPC: C12N15/70 ,  C12N1/19 ,  C12N15/10 ,  C12N15/81 ,  C12N15/90

CPC classification number: C12N15/905 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/81 ,  C12N15/1082 ,  C12Q2563/179

Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.

公开(公告)号：US11293033B2

公开(公告)日：2022-04-05

申请号：US16302079

申请日：2017-05-18

Applicant: AMYRIS, INC.

Inventor： Kevin George ,  Andrew Main ,  Chia-Hong Tsai

IPC: C12Q1/68 ,  C12N15/90 ,  C12N15/10 ,  C12N15/81

Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.