公开(公告)号：US11767533B2

公开(公告)日：2023-09-26

申请号：US17371678

申请日：2021-07-09

Applicant: AMYRIS, INC.

Inventor： Andrew Main ,  Grzegorz Wojciechowski ,  Yue Yang ,  Lishan Zhao

IPC: C12N1/16 ,  C12N15/52 ,  C12P5/00 ,  C12N1/20 ,  C12N1/14

CPC classification number: C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P5/007 ,  C12Y205/01029 ,  C12Y402/03015

Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US11085046B2

公开(公告)日：2021-08-10

申请号：US15771888

申请日：2016-10-28

Applicant: AMYRIS, INC.

Inventor： Andrew Main ,  Grzegorz Wojciechowski ,  Yue Yang ,  Lishan Zhao

IPC: C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N15/52 ,  C12P5/00

Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US12110498B2

公开(公告)日：2024-10-08

申请号：US17690429

申请日：2022-03-09

Applicant: AMYRIS, INC.

Inventor： Kevin George ,  Andrew Main ,  Chia-Hong Tsai

IPC: C12N15/70 ,  C12N1/19 ,  C12N15/10 ,  C12N15/81 ,  C12N15/90

CPC classification number: C12N15/905 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/81 ,  C12N15/1082 ,  C12Q2563/179

Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.

公开(公告)号：US11293033B2

公开(公告)日：2022-04-05

申请号：US16302079

申请日：2017-05-18

Applicant: AMYRIS, INC.

Inventor： Kevin George ,  Andrew Main ,  Chia-Hong Tsai

IPC: C12Q1/68 ,  C12N15/90 ,  C12N15/10 ,  C12N15/81

Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.