公开(公告)号：US12221641B2

公开(公告)日：2025-02-11

申请号：US17285888

申请日：2019-10-14

Applicant: AMYRIS, INC.

Inventor： Gale A. Wichmann ,  Wenzong Li ,  Sean Lund ,  Shaina J. Jackson ,  Chantal V. Garcia De Gonzalo ,  Hailley Warbington ,  Yi Xiong ,  Svetlana Alekseyevna Borisova

IPC: C12P19/56 ,  C12N1/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12R1/865

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Stevia rebaudiana kaurenoic acid hydroxylase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US12188044B2

公开(公告)日：2025-01-07

申请号：US18199278

申请日：2023-05-18

Applicant: Amyris, Inc.

Inventor： Jessica Walter

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/81

Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US20240175066A1

公开(公告)日：2024-05-30

申请号：US18551182

申请日：2022-03-18

Applicant: AMYRIS , INC.

Inventor： Nathaniel KREFMAN ,  Lauren RAETZ ,  Elizabeth ZNAMERROSKI

IPC: C12P19/44 ,  C12N1/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12P7/24

CPC classification number: C12P19/44 ,  C12N1/185 ,  C12N9/0008 ,  C12N9/1011 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/1288 ,  C12N9/88 ,  C12N15/52 ,  C12P7/24 ,  C12Y102/0103 ,  C12Y201/01114 ,  C12Y204/01126 ,  C12Y205/01054 ,  C12Y207/08007 ,  C12Y402/0101 ,  C12Y402/01118

Abstract: Provided herein are genetically modified host cells. compositions. and methods for improved production of vanillin and/or glucovanillin. The host cells. compositions. and methods described herein provide an efficient route for the heterologous production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.

公开(公告)号：US20240174987A1

公开(公告)日：2024-05-30

申请号：US18525234

申请日：2023-11-30

Applicant: AMYRIS, INC.

Inventor： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC: C12N9/10 ,  A23L2/60 ,  A23L27/30 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017 ,  A23V2002/00

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20240060056A1

公开(公告)日：2024-02-22

申请号：US18267928

申请日：2021-12-16

Applicant: Amyris, Inc.

Inventor： Jessica WALTER ,  Wenzong LI ,  Christopher F. MUGLER ,  Victoria HSIAO

IPC: C12N9/10 ,  C12P19/18 ,  C12N1/16

CPC classification number: C12N9/1051 ,  C12P19/18 ,  C12N1/165 ,  C12Y204/01146 ,  C12R2001/85

Abstract: Provided herein are variant β-1,3-N-acetylglucosaminyltransferase polypeptides capable of producing lacto-n-neotetraose, yeast cells capable of producing one or more human milk oligosaccharides, and methods of making such cells. Also, provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.

公开(公告)号：US20240026390A1

公开(公告)日：2024-01-25

申请号：US18245450

申请日：2021-09-15

Applicant: AMYRIS, INC.

Inventor： Elizabeth ZNAMEROSKI ,  Yi-Shu TAI ,  Lauren RAETZ ,  Abhishek MURARKA ,  Brandon FRIEDRIKSON

IPC: C12P7/24 ,  C12N9/88 ,  C12N9/10 ,  C12N9/12 ,  C12N9/02 ,  C12N15/52

CPC classification number: C12P7/24 ,  C12N9/88 ,  C12Y402/03004 ,  C12Y402/0101 ,  C12Y402/01118 ,  C12N9/1085 ,  C12Y205/01054 ,  C12N9/1288 ,  C12Y207/08007 ,  C12N9/0008 ,  C12Y102/0103 ,  C12N9/1051 ,  C12Y204/01126 ,  C12N9/1007 ,  C12N15/52

Abstract: Provided herein are fermentation compositions and methods for improved production of vanillin and/or glucovanillin. The compositions and methods described herein provide efficient routes for the production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.

公开(公告)号：US11866738B2

公开(公告)日：2024-01-09

申请号：US17382963

申请日：2021-07-22

Applicant: AMYRIS, INC.

Inventor： Lishan Zhao ,  Wenzong Li ,  Gale Wichmann ,  Aditi Khankhoje ,  Chantal Garcia De Gonzalo ,  Tina Mahatdejkul-Meadows ,  Shaina Jackson ,  Michael Leavell ,  Darren Platt

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017 ,  A23V2002/00

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US11802100B2

公开(公告)日：2023-10-31

申请号：US17518540

申请日：2021-11-03

Applicant: Amyris, Inc.

Inventor： Nicholas Ohler ,  Karl Fisher ,  Jin Ki Hong

IPC: C07C5/05 ,  C10L1/04 ,  C10M105/04 ,  C10M107/14 ,  C07C5/03 ,  C10M105/02

CPC classification number: C07C5/05 ,  C07C5/03 ,  C10L1/04 ,  C10M105/02 ,  C10M105/04 ,  C10M107/14 ,  C07C2521/04 ,  C07C2523/44 ,  C07C2523/75 ,  C10M2203/003 ,  C10M2203/022 ,  C10M2203/024 ,  C10M2203/0206 ,  C10M2205/083 ,  Y02P20/582 ,  C07C5/05 ,  C07C11/02

Abstract: Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C50 terpenes), methods for making the same, and methods for their use.

公开(公告)号：US11767533B2

公开(公告)日：2023-09-26

申请号：US17371678

申请日：2021-07-09

Applicant: AMYRIS, INC.

Inventor： Andrew Main ,  Grzegorz Wojciechowski ,  Yue Yang ,  Lishan Zhao

IPC: C12N1/16 ,  C12N15/52 ,  C12P5/00 ,  C12N1/20 ,  C12N1/14

CPC classification number: C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P5/007 ,  C12Y205/01029 ,  C12Y402/03015

Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US20230092433A1

公开(公告)日：2023-03-23

申请号：US17759770

申请日：2021-01-29

Applicant: AMYRIS, INC.

Inventor： Christa Lynna MARIANO ,  Ramya VISWANATHAN

IPC: A61K8/27 ,  A61K8/29 ,  A61K8/37 ,  A61K8/31 ,  A61Q17/04

Abstract: Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.