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Patent Agency Ranking
公开(公告)号：US20160040190A1
公开(公告)日：2016-02-11
申请号：US14921905
申请日：2015-10-23
Applicant: Amyris, Inc.
Inventor： Neil Stephen RENNINGER , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
Abstract: Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control one or more heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions for the maximum growth rate. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells.
Abstract translation: 提供了制备类异戊二烯的方法。 获得多个细菌或真菌宿主细胞。 这些细胞包含编码一种或多种用于制备异戊烯基焦磷酸的甲羟戊酸途径的酶的异源核酸。 一种或多种酶的表达受到一个或多个异源转录调节子的控制。 甲羟戊酸途径包括（i）使乙酰乙酰辅酶A与乙酰辅酶A缩合以形成HMG-CoA的酶，（ii）将HMG-CoA转化成甲羟戊酸的酶，（iii）将甲羟戊酸磷酸化成甲羟戊酸磷酸酯5-磷酸的酶 ，（iv）将甲羟戊酸5-磷酸转化为甲基羟基-5-焦磷酸的酶，和（v）将5-焦磷酸甲羟戊酸转化为异戊烯基焦磷酸的酶。 与最大生长速率条件相比，宿主细胞在不优选的条件下在培养基中培养。 温度保持在低于为宿主细胞提供最大比生长速率的水平。
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公开(公告)号：US09200296B2
公开(公告)日：2015-12-01
申请号：US13848045
申请日：2013-03-20
Applicant: Amyris, Inc.
Inventor： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
Abstract: Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control of at least one heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions for the maximum growth rate. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells.
Abstract translation: 提供了制备类异戊二烯的方法。 获得多个细菌或真菌宿主细胞。 这些细胞包含编码一种或多种用于制备异戊烯基焦磷酸的甲羟戊酸途径的酶的异源核酸。 一种或多种酶的表达受至少一种异源转录调节子的控制。 甲羟戊酸途径包括（i）使乙酰乙酰辅酶A与乙酰辅酶A缩合以形成HMG-CoA的酶，（ii）将HMG-CoA转化成甲羟戊酸的酶，（iii）将甲羟戊酸磷酸化成甲羟戊酸磷酸酯5-磷酸的酶 ，（iv）将甲羟戊酸5-磷酸转化为甲基羟基-5-焦磷酸的酶，和（v）将5-焦磷酸甲羟戊酸转化为异戊烯基焦磷酸的酶。 与最大生长速率条件相比，宿主细胞在不优选的条件下在培养基中培养。 温度保持在低于为宿主细胞提供最大比生长速率的水平。
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公开(公告)号：US20240035061A1
公开(公告)日：2024-02-01
申请号：US18208030
申请日：2023-06-09
Applicant: AMYRIS, INC.
Inventor： Hiroko Tsuruta , Jacob R. Lenihan , Rika Regentin
Abstract: Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.
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公开(公告)号：US12146176B2
公开(公告)日：2024-11-19
申请号：US18208030
申请日：2023-06-09
Applicant: AMYRIS, INC.
Inventor： Hiroko Tsuruta , Jacob R. Lenihan , Rika Regentin
Abstract: Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.
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公开(公告)号：US10106822B2
公开(公告)日：2018-10-23
申请号：US14921905
申请日：2015-10-23
Applicant: Amyris, Inc.
Inventor： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
Abstract: Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control one or more heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions for the maximum growth rate. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells.
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