Buffers for electrophoresis and use thereof
    1.
    发明申请
    Buffers for electrophoresis and use thereof 有权
    缓冲液用于电泳和使用

    公开(公告)号:US20030146097A1

    公开(公告)日:2003-08-07

    申请号:US10198832

    申请日:2002-07-19

    IPC分类号: G01N027/26 G01N027/447

    CPC分类号: G01N27/44747

    摘要: Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.

    摘要翻译: 各种实施方案提供例如与电泳仪器(例如毛细管电泳(CE))装置相关的缓冲液组合物和/或筛分制剂。 在各种实施方案中,缓冲组合物可以包括Bis-Tris,TAPS和/或TAPSO,以及任选的螯合剂,例如EDTA。 还描述了分离含有生物分子的样品如DNA或RNA的方法。

    High speed, high resolution compositions, methods and kits for capillary electrophoresis
    2.
    发明申请
    High speed, high resolution compositions, methods and kits for capillary electrophoresis 有权
    用于毛细管电泳的高速,高分辨率组合物,方法和试剂盒

    公开(公告)号:US20040168918A1

    公开(公告)日:2004-09-02

    申请号:US10794486

    申请日:2004-03-05

    IPC分类号: G01N027/453

    摘要: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

    摘要翻译: 本发明提供了用未经涂覆的毛细管从毛细管电泳开始高分辨率分析物的组合物,方法和试剂盒。 组合物包含筛分组分,其包含非交联的丙烯酰胺聚合物和包含至少一种不带电和非交联的水溶性二氧化硅吸附聚合物的表面相互作用组分。 提供了在毛细管电泳中使用新型组合物的方法。 还提供了包含用于新颖方法的新型组合物的试剂盒。

    Concentration and cleanup of nucleic acid samples
    3.
    发明申请
    Concentration and cleanup of nucleic acid samples 有权
    核酸样品的浓缩和清除

    公开(公告)号:US20030070925A1

    公开(公告)日:2003-04-17

    申请号:US10237537

    申请日:2002-09-09

    发明人: Karl O. Voss

    IPC分类号: G01N027/26 G01N027/447

    摘要: Methods and devices are described for concentration and cleanup of samples containing bio-molecule analytes (e.g., polynucleotides, such as DNA, RNA, PNA). Various embodiments provide for pH-mediated sample concentration and cleanup of nucleic acid samples with channel devices (e.g., cross-T format, microchannel devices).

    摘要翻译: 描述了用于浓缩和清除含有生物分子分析物(例如多核苷酸,例如DNA,RNA,PNA)的样品的方法和装置。 各种实施方案提供pH介导的样品浓度并用通道装置(例如,交叉T格式,微通道装置)清除核酸样品。