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公开(公告)号：US20240229132A1
公开(公告)日：2024-07-11
申请号：US18480850
申请日：2023-10-04
发明人： Jia Guo , Joshua LaBaer
IPC分类号： C12Q1/6874 , C12Q1/6876
CPC分类号： C12Q1/6874 , C12Q1/6876 , C12Q2563/131
摘要： Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable fluorescent streptavidin. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.
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公开(公告)号：US11814677B2
公开(公告)日：2023-11-14
申请号：US17138287
申请日：2020-12-30
发明人： Jia Guo , Joshua LaBaer
IPC分类号： C12Q1/68 , C12Q1/6874 , C12Q1/6876
CPC分类号： C12Q1/6874 , C12Q1/6876 , C12Q2563/131
摘要： Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable hapten linked targeting agents and cleavable detectably-labeled hapten-binding agents. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.
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公开(公告)号：US11512344B2
公开(公告)日：2022-11-29
申请号：US16300148
申请日：2017-04-25
发明人： Jia Guo
IPC分类号： C12Q1/68 , C12Q1/6841 , G01N21/64 , C12Q1/6825
摘要： Provided herein are high-throughput, high-quality methods of consecutive in situ hybridization for analysis of the genome and/or transcriptome in an individual cell with single-molecule sensitivity. In particular, provided herein are methods comprising visualizing individual genomic loci or transcripts as single detectable signals (e.g., fluorescent spots) which remain in place during consecutive hybridization. In each cycle of consecutive hybridization, detectably labeled probes hybridize to the probe used in the previous cycle, and also introduce the binding sites for the probe of the following cycle. Through consecutive cycles of probe hybridization, imaging, and signal removal, different genomic loci or RNA species can be identified by unique detectable signal profiles (e.g., fluorescent spots with unique color sequences). The number of varied color sequences increases exponentially with the number of hybridization cycles, which enables the genome or transcriptome-wide analysis.
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公开(公告)号：US20210198735A1
公开(公告)日：2021-07-01
申请号：US17138287
申请日：2020-12-30
发明人： Jia Guo , Joshua LaBaer
IPC分类号： C12Q1/6874 , C12Q1/6876
摘要： Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable hapten linked targeting agents and cleavable detectably-labeled hapten-binding agents. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.
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公开(公告)号：US20190144932A1
公开(公告)日：2019-05-16
申请号：US16300148
申请日：2017-04-25
发明人： Jia Guo
IPC分类号： C12Q1/6841 , G01N21/64 , C12Q1/6825
摘要： Provided herein are high-throughput, high-quality methods of consecutive in situ hybridization for analysis of the genome and/or transcriptome in an individual cell with single-molecule sensitivity. In particular, provided herein are methods comprising visualizing individual genomic loci or transcripts as single detectable signals (e.g., fluorescent spots) which remain in place during consecutive hybridization. In each cycle of consecutive hybridization, detectably labeled probes hybridize to the probe used in the previous cycle, and also introduce the binding sites for the probe of the following cycle. Through consecutive cycles of probe hybridization, imaging, and signal removal, different genomic loci or RNA species can be identified by unique detectable signal profiles (e.g., fluorescent spots with unique color sequences). The number of varied color sequences increases exponentially with the number of hybridization cycles, which enables the genome or transcriptome-wide analysis.
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公开(公告)号：US20230399683A1
公开(公告)日：2023-12-14
申请号：US17975384
申请日：2022-10-27
发明人： Jia Guo
IPC分类号： C12Q1/6841 , G01N21/64 , C12Q1/6825
CPC分类号： C12Q1/6841 , G01N21/64 , C12Q1/6825 , G01N21/6428 , G01N2021/6439
摘要： Provided herein are high-throughput, high-quality methods of consecutive in situ hybridization for analysis of the genome and/or transcriptome in an individual cell with single-molecule sensitivity. In particular, provided herein are methods comprising visualizing individual genomic loci or transcripts as single detectable signals (e.g., fluorescent spots) which remain in place during consecutive hybridization. In each cycle of consecutive hybridization, detectably labeled probes hybridize to the probe used in the previous cycle, and also introduce the binding sites for the probe of the following cycle. Through consecutive cycles of probe hybridization, imaging, and signal removal, different genomic loci or RNA species can be identified by unique detectable signal profiles (e.g., fluorescent spots with unique color sequences). The number of varied color sequences increases exponentially with the number of hybridization cycles, which enables the genome or transcriptome-wide analysis.
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