公开(公告)号：US10907145B2

公开(公告)日：2021-02-02

申请号：US15915909

申请日：2018-03-08

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Kaushal Rege ,  Kevin Lin ,  Sudhakar Godeshala ,  Taraka Sai Pavan Grandhi

IPC: C12N15/10 ,  B01D15/38 ,  B01J20/291

Abstract: Ligands and methods for selectively binding hypermethylated DNA from a sample. The ligands include a CG-region binding molecule-conjugated resin derived from the aminoglycoside antibiotic amikacin. Furthermore, the CG-region binding molecule may be conjugated to the resin with a crosslinker and/or may be modified with one or more of long chain and short chain alkyl, aryl, piperazinyl, piperidyl, and pyrrolidyl groups. Such ligands are used in methods for contacting a sample to thereby selectively bind hypermethylated DNA.