公开(公告)号：US20220257659A1

公开(公告)日：2022-08-18

申请号：US17723209

申请日：2022-04-18

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Evangelia Kokalaki ,  Shaun Cordoba ,  Shimobi Onuoha ,  Simon Thomas ,  Biao Ma ,  Mathieu Ferrari

IPC分类号： A61K35/17 ,  A61P35/00 ,  C07K16/28

摘要： The present invention provides a chimeric antigen receptor (CAR) which binds a target antigen having a bulky extracellular domain, wherein the CAR comprises a Fab antigen binding domain. The present invention also provides nucleic acid sequences and constructs encoding such a CAR, cells expressing such a CAR and their therapeutic uses.

公开(公告)号：US20210113618A1

公开(公告)日：2021-04-22

申请号：US17054670

申请日：2019-05-15

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Evangelia Kokalaki ,  Shaun Cordoba ,  Shimobi Onuoha ,  Simon Thomas ,  Biao Ma ,  Mathieu Ferrari

IPC分类号： A61K35/17 ,  C07K16/28 ,  A61P35/00

摘要： The present invention provides a chimeric antigen receptor (CAR) which binds a target antigen having a bulky extracellular domain, wherein the CAR comprises a Fab antigen binding domain. The present invention also provides nucleic acid sequences and constructs encoding such a CAR, cells expressing such a CAR and their therapeutic uses.

公开(公告)号：US11180553B2

公开(公告)日：2021-11-23

申请号：US16310121

申请日：2017-06-15

申请人： AUTOLUS LIMITED

发明人： Shimobi Onuoha ,  Martin Pulé ,  Simon Thomas ,  Shaun Cordoba ,  Evangelia Kokalaki

IPC分类号： C07K16/28 ,  C07K14/725 ,  A61P35/02 ,  A61P35/00 ,  A61K35/17 ,  C07K14/705 ,  A61K38/00

摘要： The present invention provides a chimeric antigen receptor (CAR) which binds human CD22, having an antigen-binding domain which comprises a) a heavy chain variable region (VH) having complementarity determining regions (CDRs) with the following sequences: CDR1—NYWIH (SEQ ID No. 1); CDR2—GINPGNNYATYRRKFQG (SEQ ID No. 2) CDR3—EGYGNYGAWFAY (SEQ ID No. 3); and b) a light chain variable region (VL) having CDRs with the following sequences: CDR1—RSSQSLANSYGNTFLS (SEQ ID No. 4); CDR2—GISNRFS (SEQ ID No. 5) CDR3—LQGTHQPYT (SEQ ID No. 6). The present invention also provides a cell comprising such a CAR and the use of such a cell to treat cancer.

公开(公告)号：US20190177412A1

公开(公告)日：2019-06-13

申请号：US16310121

申请日：2017-06-15

申请人： AUTOLUS LIMITED

发明人： Shimobi Onuoha ,  Martin Pulé ,  Simon Thomas ,  Shaun Cordoba ,  Evangelia Kokalaki

IPC分类号： C07K16/28 ,  C07K14/705 ,  A61K35/17 ,  A61P35/00 ,  A61P35/02 ,  C07K14/725

摘要： The present invention provides a chimeric antigen receptor (CAR) which binds human CD22, having an antigen-binding domain which comprises a) a heavy chain variable region (VH) having complementarity determining regions (CDRs) with the following sequences: CDR1—NYWIH (SEQ ID No. 1); CDR2—GINPGNNYATYRRKFQG (SEQ ID No. 2) CDR3—EGYGNYGAWFAY (SEQ ID No. 3); and b) a light chain variable region (VL) having CDRs with the following sequences: CDR1—RSSQSLANSYGNTFLS (SEQ ID No. 4); CDR2—GISNRFS (SEQ ID No. 5) CDR3—LQGTHQPYT (SEQ ID No. 6). The present invention also provides a cell comprising such a CAR and the use of such a cell to treat cancer.

公开(公告)号：US20230233606A1

公开(公告)日：2023-07-27

申请号：US17784408

申请日：2020-12-18

申请人： AUTOLUS LIMITED

发明人： Shaun Cordoba ,  Evangelia Kokalaki ,  Thomas Grothier ,  Shimobi Onuoha ,  Simon Thomas ,  Martin Pulé

IPC分类号： A61K35/17 ,  C12N9/12 ,  C12N5/0783 ,  C07K16/28 ,  C07K14/705 ,  C07K14/725

CPC分类号： A61K35/17 ,  C12N9/12 ,  C12Y207/10002 ,  C12N5/0636 ,  C07K16/2803 ,  C07K14/70578 ,  C07K14/7051 ,  C07K2317/622 ,  C07K2319/03 ,  C07K2319/33

摘要： The present invention provides cell which co-expresses a chimeric antigen receptor (CAR) and a dominant negative C-terminal Src kinase (dnCSK). The present invention also provides nucleic acid constructs, vectors and methods for making such a cell and the use of such a cell in the treatment of diseases such as cancer by adoptive immunotherapy.

公开(公告)号：US11590170B2

公开(公告)日：2023-02-28

申请号：US17723209

申请日：2022-04-18

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Evangelia Kokalaki ,  Shaun Cordoba ,  Shimobi Onuoha ,  Simon Thomas ,  Biao Ma ,  Mathieu Ferrari

IPC分类号： A61K35/17 ,  A61P35/00 ,  C07K16/28

摘要： The present invention provides a chimeric antigen receptor (CAR) which binds a target antigen having a bulky extracellular domain, wherein the CAR comprises a Fab antigen binding domain. The present invention also provides nucleic acid sequences and constructs encoding such a CAR, cells expressing such a CAR and their therapeutic uses.

公开(公告)号：US20200338124A1

公开(公告)日：2020-10-29

申请号：US16613364

申请日：2018-05-14

申请人： AUTOLUS LIMITED

发明人： Shaun Cordoba ,  Evangelia Kokalaki ,  Martin Pulé ,  Simon Thomas ,  Shimobi Onuoha

IPC分类号： A61K35/17 ,  C07K16/28 ,  C07K14/725 ,  C07K14/705 ,  C12N15/86

摘要： The present invention provides a cell which co-expresses a first chimeric antigen receptor (CAR) and second CAR wherein the first CAR comprises an activating endodomain and the second CAR comprises an inhibitory endodomain, wherein the inhibitory endodomain comprises C-terminal Src Kinase (CSK).

公开(公告)号：US20190309046A1

公开(公告)日：2019-10-10

申请号：US16463258

申请日：2017-11-27

申请人： AUTOLUS LIMITED

发明人： Shaun Cordoba ,  Evangelia Kokalaki ,  Vania Baldan ,  Simon Thomas ,  Maria Stavrou

IPC分类号： C07K14/73 ,  C07K14/705 ,  C07K16/28 ,  C12N9/12 ,  C12N9/16

摘要： The present invention provides signal transduction modifying protein which comprises a domain which binds a phosphorylated immunoreceptor tyrosine-based inhibition motif (pITIM). The signal transduction modifying protein lacks a functional phosphatase domain. The present invention also provides cells which express such a signal transduction modifying protein, and cells which co-express such a signal transduction modifying protein together with a chimeric antigen receptor (CAR).

公开(公告)号：US11963981B2

公开(公告)日：2024-04-23

申请号：US17054670

申请日：2019-05-15

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Evangelia Kokalaki ,  Shaun Cordoba ,  Shimobi Onuoha ,  Simon Thomas ,  Biao Ma ,  Mathieu Ferrari

IPC分类号： A61K35/17 ,  A61P35/00 ,  C07K16/28

CPC分类号： A61K35/17 ,  A61P35/00 ,  C07K16/2803 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/76

摘要： The present invention provides a chimeric antigen receptor (CAR) which binds a target antigen having a bulky extracellular domain, wherein the CAR comprises a Fab antigen binding domain. The present invention also provides nucleic acid sequences and constructs encoding such a CAR, cells expressing such a CAR and their therapeutic uses.

公开(公告)号：US20210030798A1

公开(公告)日：2021-02-04

申请号：US16967377

申请日：2019-02-04

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Shaun Cordoba ,  Shimobi Onuoha ,  Simon Thomas ,  Evangelia Kokalaki ,  Ram Jha

IPC分类号： A61K35/17 ,  C07K14/725 ,  C07K16/28 ,  C07K14/705

摘要： The present invention provides a chimeric receptor which binds a target antigen on a target cell, which comprises: a first antigen binding domain which binds a first epitope of the target antigen, a second antigen binding domain which binds a second epitope of the target antigen; a transmembrane domain; and an intracellular signalling domain.