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    Plasmid system
    3.
    发明授权
    Plasmid system 有权

    公开(公告)号：US11965173B2

    公开(公告)日：2024-04-23

    申请号：US16772216

    申请日：2018-12-14

    申请人： AUTOLUS LIMITED

    发明人： Martin Pulé ,  Paulo Fernandes ,  Hanna Kymalainen ,  Ekaterini Kotsopoulou

    IPC分类号： C12N15/86 ,  C12N15/85

    CPC分类号： C12N15/86 ,  C12N15/85 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2800/107

    摘要： There is provided a plasmid system for transfection into a cell to create a producer cell, the system comprising: a. a helper plasmid comprising a first nucleotide sequence encoding Murine leukemia virus (MLV)-derived Gag and Pol poly-proteins; b. an envelope plasmid comprising a second nucleotide sequence encoding an Env protein; c. a genome plasmid comprising a third nucleotide sequence encoding a retroviral genome, wherein the first nucleotide sequence is codon-shuffled to remove any significant regions of homology with the third nucleotide sequence; and wherein the second nucleotide sequence is codon-optimised for expression in the producer cell.

    PLASMID SYSTEM
    6.
    发明公开
    PLASMID SYSTEM 审中-公开

    公开(公告)号：US20230242937A1

    公开(公告)日：2023-08-03

    申请号：US16772216

    申请日：2018-12-14

    申请人： AUTOLUS LIMITED

    发明人： Martin Pulé ,  Paulo Fernandes ,  Hanna Kymalainen ,  Ekaterini Kotsopulou

    IPC分类号： C12N15/86 ,  C12N15/85

    CPC分类号： C12N15/86 ,  C12N15/85 ,  C12N2740/13052 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2800/107

    摘要： There is provided a plasmid system for transfection into a cell to create a producer cell, the system comprising: a. a helper plasmid comprising a first nucleotide sequence encoding Murine leukemia virus (MLV)-derived Gag and Pol poly-proteins; b. an envelope plasmid comprising a second nucleotide sequence encoding an Env protein; c. a genome plasmid comprising a third nucleotide sequence encoding a retroviral genome, wherein the first nucleotide sequence is codon-shuffled to remove any significant regions of homology with the third nucleotide sequence; and wherein the second nucleotide sequence is codon-optimised for expression in the producer cell.

    Engineered Cytolytic Immunecell
    8.
    发明申请
    Engineered Cytolytic Immunecell 有权

    公开(公告)号：US20230090553A1

    公开(公告)日：2023-03-23

    申请号：US17823003

    申请日：2022-08-29

    申请人： AUTOLUS LIMITED

    发明人： Shaun Cordoba ,  Shimobi Onuoha ,  Alexander Kinna ,  Simon Thomas ,  Ram Jha ,  Martin Pulé

    IPC分类号： C12N15/85 ,  A61K35/17 ,  C07K14/725 ,  C12N5/0783 ,  G01N33/50

    摘要： The present invention relates to engineered a cytolytic immune cell comprising: i) a releasable protein which comprises a polypeptide of interest (POI) and a first interaction domain; and ii) a retention protein which is retained within an intracellular compartment of the cell and comprises a second interaction domain which binds to the first protein interaction domain, wherein binding between the first protein interaction domain and second protein interaction domain is disrupted by the presence of an agent, such that in the absence of the agent, the first protein interaction domain and second protein interaction domain bind and result in retention of the POI within an intracellular compartment; whereas in the presence of the agent, the first protein interaction domain and second protein interaction do not bind and the POI is released from the intracellular compartment and expressed at the cell surface or secreted by the cell.

    Nucleic acid construct
    9.
    发明授权
    Nucleic acid construct 有权

    公开(公告)号：US11530420B2

    公开(公告)日：2022-12-20

    申请号：US15568859

    申请日：2016-04-26

    申请人： AUTOLUS LIMITED

    发明人： Martin Pulé ,  Shaun Cordoba

    IPC分类号： C12N15/00 ,  C12N15/86 ,  C07K14/705 ,  C07K16/28 ,  C12N15/85

    摘要： The present invention provides a nucleic acid construct comprising the following structure: A-X-B in which A and B are nucleic acid sequences encoding a first and a second polypeptide of interest (POI); and X is a nucleic acid sequence which encodes a cleavage site, wherein either the first or second POI is a transmembrane protein which comprises an intracellular retention signal.