公开(公告)号：US11965173B2

公开(公告)日：2024-04-23

申请号：US16772216

申请日：2018-12-14

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Paulo Fernandes ,  Hanna Kymalainen ,  Ekaterini Kotsopoulou

IPC分类号： C12N15/86 ,  C12N15/85

CPC分类号： C12N15/86 ,  C12N15/85 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2800/107

摘要： There is provided a plasmid system for transfection into a cell to create a producer cell, the system comprising: a. a helper plasmid comprising a first nucleotide sequence encoding Murine leukemia virus (MLV)-derived Gag and Pol poly-proteins; b. an envelope plasmid comprising a second nucleotide sequence encoding an Env protein; c. a genome plasmid comprising a third nucleotide sequence encoding a retroviral genome, wherein the first nucleotide sequence is codon-shuffled to remove any significant regions of homology with the third nucleotide sequence; and wherein the second nucleotide sequence is codon-optimised for expression in the producer cell.

公开(公告)号：US20230242937A1

公开(公告)日：2023-08-03

申请号：US16772216

申请日：2018-12-14

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Paulo Fernandes ,  Hanna Kymalainen ,  Ekaterini Kotsopulou

IPC分类号： C12N15/86 ,  C12N15/85

CPC分类号： C12N15/86 ,  C12N15/85 ,  C12N2740/13052 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2800/107

摘要： There is provided a plasmid system for transfection into a cell to create a producer cell, the system comprising: a. a helper plasmid comprising a first nucleotide sequence encoding Murine leukemia virus (MLV)-derived Gag and Pol poly-proteins; b. an envelope plasmid comprising a second nucleotide sequence encoding an Env protein; c. a genome plasmid comprising a third nucleotide sequence encoding a retroviral genome, wherein the first nucleotide sequence is codon-shuffled to remove any significant regions of homology with the third nucleotide sequence; and wherein the second nucleotide sequence is codon-optimised for expression in the producer cell.