公开(公告)号：US20140329279A1

公开(公告)日：2014-11-06

申请号：US14270675

申请日：2014-05-06

Applicant: AbbVie Inc.

Inventor： Min Wang ,  Patrick M. Hossler ,  Sean W. McDermott ,  Christopher Racicot ,  Kofi Chemfe ,  John Fann

IPC: C12N5/00 ,  C07K16/24

CPC classification number: C07K16/241 ,  A61K39/39591 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0043 ,  C12N5/005 ,  C12N2501/999 ,  C12N2510/02

Abstract: Supplementation of the bioflavonoids such as epigallocatechin gallate, rutin, naringin, or genistein into mammalian cell culture media are shown to be effective in reduction of acidic species variants on recombinant antibodies. The demonstrated reduction in acidic species through the use of bioflavonoids, facilitates the manufacturing of a less heterogeneous product with potential improvements in antibody structure and function.

Abstract translation: 显示在哺乳动物细胞培养基中补充生物类黄酮如表没食子儿茶素没食子酸酯，芦丁，柚皮苷或染料木素可有效减少重组抗体上的酸性物种变体。 通过使用生物类黄酮证明酸性物质的减少有利于制造具有潜在改进抗体结构和功能的较不均匀的产品。

公开(公告)号：US09416181B2

公开(公告)日：2016-08-16

申请号：US14270675

申请日：2014-05-06

Applicant: AbbVie Inc.

Inventor： Min Wang ,  Patrick M. Hossler ,  Sean W. McDermott ,  Christopher Racicot ,  Kofi Chemfe ,  John Fann

IPC: C12N5/00 ,  C07K16/24 ,  A61K39/395 ,  C07K16/00

CPC classification number: C07K16/241 ,  A61K39/39591 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0043 ,  C12N5/005 ,  C12N2501/999 ,  C12N2510/02

Abstract: Supplementation of the bioflavonoids such as epigallocatechin gallate, rutin, naringin, or genistein into mammalian cell culture media are shown to be effective in reduction of acidic species variants on recombinant antibodies. The demonstrated reduction in acidic species through the use of bioflavonoids, facilitates the manufacturing of a less heterogeneous product with potential improvements in antibody structure and function.

Abstract translation: 显示在哺乳动物细胞培养基中补充生物类黄酮如表没食子儿茶素没食子酸酯，芦丁，柚皮苷或染料木素可有效减少重组抗体上的酸性物种变体。 通过使用生物类黄酮证明酸性物质的减少有利于制造具有潜在改进抗体结构和功能的较不均匀的产品。